摘要
目的:利用1H-NMR代谢组学技术研究帕金森抑郁患者血清代谢标记物变化及柴胡加龙骨牡蛎汤的影响。方法:收集帕金森抑郁患者在服用药物前及其单纯服用多巴丝肼片12周,在多巴丝肼片基础上加上柴胡加龙骨牡蛎汤服用12周及空白对照组(健康人)分别10例的血清样本,通过主成分分析(PCA)和正交偏最小二乘判别分析(OPLS-DA)分析两者的差异血清代谢产物谱。结果:与健康对照组比较,帕金森抑郁患者组谷氨酰胺、谷氨酸、胍基乙酸含量显著增加(P<0.05),经两种方法治疗12周后,谷氨酰胺、谷氨酸、胍基乙酸的含量显著减少(P<0.05);与单纯服用多巴丝肼片组比较,柴胡加龙骨牡蛎汤组谷氨酰胺、谷氨酸、胍基乙酸含量减少显著(P<0.05)。结论:柴胡加龙骨牡蛎汤可以调整帕金森抑郁患者的氨基酸代谢和能量代谢。
Objective:To study the serum metabolic markers changes of Parkinson’s disease with depression and the effects of Chaihu Jia Longgu Muli Decoction by 1H-NMR metabolomics technology.Methods:Ten patients with Parkinson’s disease with depression were collected for 12 weeks before taking the drug and taking Dopaszine tablets,the other 10 patients based on the Dopaszine tablets plus Chaihu Jia Longgu Muli Decoction for 12 weeks.Blank control group(healthy person)sample was also ten.The serum samples of the patients or healthy person were analyzed for differential serum metabolite profiles by principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA).Results:Compared with the lhealthy group,the levels of glutamine,glutamic acid and guanidinoacetic acid in Parkinson’s disease with depression patients group increased significantly(P<0.05).After 12 weeks of treatment,the contents of glutamine,glutamic acid and guanidinoacetic acid were significantly reduced(P<0.05).Compared with the group taking Dopasazine alone,the content of glutamate,glutamic acid and guanidinoacetic acid in the group of Chaihu Jia Longgu Muli Decoction was significantly reduced(P<0.05).Conclusion:Chaihu Jia Longgu Muli Decoction could adjust the amino acid metabolism and energy metabolism of Parkinson’s disease with depression patients.
作者
秦劭晨
王爱梅
李若瑜
夏天
周珊
QIN Shaochen;WANG Aimei;LI Ruoyu;XIA Tian;ZHOU Shan(The Affiliated Hospital of Shanxi University of Chinese Medicine,Taiyuan,Shanxi 030024;Guangzhou University of Chinese Medicine,Guangzhou,Guangdong 510006)
出处
《中国中医药科技》
CAS
2021年第2期180-183,共4页
Chinese Journal of Traditional Medical Science and Technology
基金
山西中医药大学科技创新能力培育计划项目(2019PY-051)
山西省卫生和计划生育委员会科研课题(201601119)。
关键词
帕金森抑郁
多巴丝肼
柴胡加龙骨牡蛎汤
血清代谢组学
Parkinson’s disease with depression
Chaihu Jia Longgu Muli Decoction
Dopasazine
serum metabolomics
作者简介
通讯作者:王爱梅,E-mail:w15803439738@qq.com。
