摘要
目的研究丹酚酸B(SAB)能否减轻脂多糖(LPS)诱导的急性肺损伤。方法将48只小鼠随机分为对照组、模型组(气管滴注LPS)、SAB低/中/高剂量干预组、莱菔硫烷阳性对照组,检测肺湿/干重比(W/D)值;检测肺泡灌洗液(BALF)中蛋白浓度;HE染色法评价肺组织病理损伤;ELISA检测BALF中TNF-α、IL-1β和IL-6的含量,检测肺组织MPO、SOD的活性和MDA含量;Western blot检测肺组织中Nrf2、NQO1、HO1、Keap1、NF-κB及p-NF-κB的蛋白表达水平。结果与对照组相比,模型组小鼠肺组织产生病理性变化,W/D值、BALF中蛋白质浓度、炎性细胞因子含量明显升高(P<0.05),急性肺损伤模型建立成功。与模型组比较,SAB干预组的各指标值发生显著变化(P<0.05);减轻LPS诱导的急性肺损伤小鼠氧化应激,且呈浓度依赖性增强,同时降低Keap1的表达(P<0.01);升高Nrf2、NQO1和HO1的表达(P<0.01);抑制LPS诱导的NF-κB的表达和NF-κB的磷酸化。结论SAB可以减轻LPS诱导的小鼠急性肺损伤。
Objective To study whether salvianolic acid B(SAB)can reduce lipopolysaccharide(LPS)-induced acute lung injury.MethodsFourty-eight mice were randomly divided into control group,model group(intratracheal instillation of LPS),SAB low/medium/high dose intervention group,and sulforaphane positive control group.The lung wet/dry weight ratio(W/D)was recorded;The protein concentration of alveolar lavage fluid(BALF)was detected;Lung tissue pathological damage was evaluated by HE staining and microoscopy;TNF-α,IL-1βand IL-6 in BALF were detected by ELISA.Western blot was used to detect the protein expression of Nrf2,NQO1,HO1,Keap1,NF-κB and p-NF-κB in lung tissue.Results Compared with the control group,pathological changes were observed in the lung tissue of the model group,W/D,protein concentration in BALF and inflammatory cytokine content were significantly increased(P<0.05).Compared with the model group,SAB intervention group had significant changes(P<0.05)that alleviated the oxidative stress induced by LPS,increased the expression of Keap1(P<0.01),increased the expression of Nrf2,NQO1 and HO1(P<0.01),and inhibited the activation of NF-κB and p-NF-κBinduced by LPS.Conclusions SAB can alleviate LPS-induced acute lung injury of mice.
作者
李玉婷
罗乐
尹素娟
朱小兰
LI Yu-ting;LUO Le;YIN Su-juan;ZHU Xiao-lan(College of Medicine,Yongzhou Vocational Technical College, Yongzhou 425006;School of Pharmaceutical Sciences,Central South University,Changsha 410205,China)
出处
《基础医学与临床》
2021年第3期376-381,共6页
Basic and Clinical Medicine
基金
湖南省教育厅科学研究项目(18C1748)。
关键词
丹酚酸B
急性肺损伤
氧化应激
salvianolic acid B
acute lung injury
oxidative stress
作者简介
通信作者:罗乐,le67@qq.com。
