摘要
目的 探讨骨髓充质干细胞(bone mesenchymal stem cell,BMSC)对急性肺损伤(acute lung injury,ALI)幼鼠炎症因子IL-1β、IL-10的影响.方法 SPF级雄性SD幼年鼠72只,随机分为3组:对照组、疾病模型组(模型组)、疾病模型+ BMSC(治疗组),各组再按时段随机分为4个亚组.建立幼年鼠ALI模型,治疗组注射BMSC,模型组、对照组注射等体积培养基(DMEM).观察肺组织病理形态变化,检测W/D比值和支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中性粒细胞计数及蛋白质含量,检测IL-1β、IL-10含量的变化.结果 与对照组、治疗组相比,模型组各个时间段的BALF中性粒细胞数量、总蛋白的含量、肺组织W/D比值均明显升高,差异具有统计学意义(P<0.05).治疗组肺组织损伤程度较模型组相比明显减轻.血清中的IL-1β和IL-10含量在不同时间段均呈降低状态,48h时段与6h时段存在显著差异,具有统计学意义(P<0.05).模型组幼年鼠肺组织IL-1β蛋白大量表达于肺泡上皮细胞胞浆及细胞壁上;而治疗组蛋白表达强度明显下降.模型组各个时间段的肺组织IL-1β、IL-10蛋白含量均明显较高,差异具有统计学意义(P<0.05).结论 BMSC可对ALI幼鼠炎症因子IL-1β、IL-10产生重要影响,BMSC能够减轻肺损伤炎症反应,并参与了肺损伤的修复.
Objective To investigate the effects of bone mesenchymal stem cells(BMSC)on the expression level of inflammatory factors IL-1beta and IL-10 from young rats with acute lung injury(ALI).Methods Seventy-two SPF male SD young rats were randomly divided into three groups:control group,disease model group(model group),disease model+BMSC(treatment group).Each group was then randomly divided into four subgroups according to the treatment phages.The juvenile rat ALI models were established.BMSC was injected into the treatment group,and DMEM was injected into the model and the control groups.Then,the pathological changes of lung tissues were observed.The W/D ratio,the neutrophil count and protein content in bronchoalveolar lavage fluid(BALF)were measured.The changes of IL-1beta and IL-10 contents were detected.Results Compared with the control group and the treatment group,the number of neutrophils,total protein content and W/D ratio of BALF lung tissue in the model group increased significantly in each period(P<0.05).The degree of lung injury in the treatment group was significantly weaker than that in the model group.The levels of IL-1beta and IL-10 in serum decreased in different treatment periods(48 hours vs 6 hours,P<0.05).In model group,IL-1beta was abundantly expressed in the cytoplasm and wall of alveolar epithelial cells,while in treatment group,the expression intensity of IL-1 beta protein was significantly decreased.The levels of IL-1 beta and IL-10 protein in lung tissues of model group were significantly higher than those of control group(P<0.05).Conclusion BMSC has an important effect on the expression of inflammatory factors IL-1beta and IL-10 in ALI young rates.BMSC can alleviate the inflammatory reaction of long injury and participate in the repair of lung iniury.
作者
刘凯
李玖军
孙鹏
李倩影
Liu Kai;Li Jiujun;Sun Peng;Li Qianying(Department of PICU,Shengjing Hospital of China Medical University,Shenyang 110004,China)
出处
《国际儿科学杂志》
2018年第12期973-977,F0003,共6页
International Journal of Pediatrics
基金
辽宁省自然科学基金(2014021077)Natural Science Foundation of Liaoning Province(2014021077).
关键词
骨髓间充质干细胞
急性肺损伤
幼鼠
IL-1Β
IL-10
Bone marrow mesenchymal stem cell
Acute lung injury
Young mice
Interleukins-1 beta
Interleukin-10
作者简介
通信作者:李玖军,Email:lijj@sj-hosipital.org
