摘要
为了探讨人参茎叶总皂苷(ginseng stem-leaf saponins,GSLS)联合亚硒酸钠(Na2SeO3,简称Se)对伪狂犬病病毒(PRV)灭活疫苗免疫的增强作用,本试验给小鼠口服GSLS后,接种添加了Se的PRV灭活疫苗,并检测免疫后小鼠血清中PRV gB抗体及其亚类(IgG1和IgG2a)水平、淋巴细胞增殖水平、淋巴细胞分泌细胞因子(IFN-γ、IL-2、IL-4和IL-6)水平以及野毒(fPRV)攻毒后小鼠的存活率。结果显示,与仅仅免疫PRV灭活疫苗的小鼠相比,口服GSLS联合疫苗中添加Se(GSLS-Se)能够显著提高小鼠血清中PRV gB抗体及其亚类(IgG1和IgG2a)水平,淋巴细胞增殖水平和细胞因子(IFN-γ、IL-2和IL-6)水平。此外,GSLS-Se还提高了免疫小鼠对野毒感染的抵抗力。结果表明,GSLS-Se对PRV灭活疫苗具有免疫增强作用,值得在本动物上进一步研究。
Previous studies have demonstrated that oral administration of ginseng stem-leaf saponins(GSLS)can enhance the immune response to vaccination.To investigate the immune potentiating effect of GSLS in combination with sodium selenite(Na2SeO3,Se)on the immune response to an inactivated pseudorabies virus(PRV)vaccine,mice were immunized with PRV vaccine supplemented with Se after oral administration of GSLS.Results showed that oral administration of GSLS in combination with supplement of Se in vaccine stimulated significantly higher serum gB-specific antibody responses(IgG,IgG1 and IgG2 a),increased lymphocyte proliferation as well as higher production of cytokines(IFN-γ,IL-2 and IL-6)by splenocytes when compared with the control.More notably,GSLS-Se conferred a much higher resistance to fPRV challenge in mice immunized with a PRV vaccine.Therefore,GSLS-Se has immune potentiating effect on the immune response and deserves further study in domestic animals.
作者
MAQBOOL Babar
王勇
崔雪梅
袁丽佳
胡松华
MAQBOOL Babar;WANG Yong;CUI Xuemei;YUAN Lijia;HU Songhua(College of Animal Sciences,Zhejiang University,Hangzhou 310058,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2021年第2期231-235,272,共6页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(31672598)。
关键词
人参茎叶总皂苷
亚硒酸钠
伪狂犬病毒灭活疫苗
佐剂
ginseng stem-leaf saponins
sodium selenite
inactivated pseudorabies virus vaccine
adjuvant
作者简介
共同第1作者:MAQBOOL Babar(1989-),男,博士;共同第1作者:王勇(1988-),男,博士;通讯作者:胡松华,E-mail:songhua@zju.edu.cn。
