摘要
采用发光二极管(light-emitting diode,LED)精确调制光谱能量,研究不同光质对水稻秧苗素质(株高、根长和鲜干重)和生理特征(内源激素、抗氧化酶、可溶性蛋白和丙二醛)的影响,探讨生理特征与秧苗素质和补光移栽后产量的耦合关系。水稻秧苗分别在5种光质下进行补光照射:R (100%红光),B (100%蓝光),RB (80%红光+20%蓝光)RBG_(12.5)(62.5%红光+25%蓝光+12.5%绿光),RBG_(25)(50%红光+25%蓝光+25%绿光),光量子通量均为1 000μmol·m^(-2)·s^(-1),照射30 d (6 h/d)后采样,并插秧至稻田,以无补光作为对照(CK)。与CK相比,LED补充光源显著(P<0.05)影响水稻秧苗的株高、根长和鲜干重。株高和地上部分干重最大值分别出现在R和RBG_(12.5)处理,而根长和地下部分干重的最大值均出现在RBG_(12.5)处理。LED补充光源对内源激素、抗氧化酶、可溶性蛋白和丙二醛的影响程度因光质和植物部位而异。与CK相比,RBG_(12.5)二显著(P<0.05)提高地上部生长素(IAA)含量和可溶性蛋白含量,B显著(P<0.05)提高地上部超氧化歧化酶(SOD)活性、过氧化物酶(POD)活性和丙二醛(MDA)含量;RBG_(12.5)和RBG_(25)显著(P<0.05)提高地下部IAA含量,RBC_(25)显著(P<0.05)提高地下部可溶性蛋白含量和MDA含量。补光移栽后水稻产量的变化趋势与光质对水稻秧苗壮苗指数的影响趋势一致:RBG_(12.5)> RBG25> RB> B> R> CK。逐步回归分析发现,壮苗指数与地上部赤霉素(GA_3)含量和IAA含量及地下部IAA含量和SOD活性显著正相关;补光移栽后产量与秧苗地上部IAA含量及地下部IAA含量和过氧化氢酶(CAT)活性显著正相关。LED补充光源通过调节水稻秧苗生理特征影响其素质,且影响效应延续至水稻成熟期。RBG_(12.5)光源更利于培育水稻壮苗和水稻增产,适宜用作工厂化育秧的补充光源。
The paper investigated the responses of rice(Oryza sat iva L.)seedling parameters(plant height,root length,and fresh and dry weight)and physiological characteristics(endogenous hormones,antioxidant enzymes,soluble protein and malondialdehyde)to the different light quality of supplemental light-emitting diodes(LEDs),and their after-effects on the grain yield.Rice seedlings were nursed at 1000μmol(photons)·m-2·s-1 in a greenhouse with 30 day continuous supplemental lighting(6 h·d-1)from different light sources,including no supplemental light(CK),100%red(R),100%blue(B),80%red+20%blue(RB),62.5%red+25%blue+12.5%green(RBG 12.5)and 50%red+25%blue+25%green(RBG 25),and then were transplanted into paddy field.The results showed that the plant height,root length,fresh and dry weight of rice seedlings were significantly(P<0.05)varied with the supplemental light sources.The highest plant height and the greatest aboveground dry weight were presented in R and RBG 12.5,respectively,while the longest root length and the greatest belowground dry weight were observed in RBG 12.5.Additionally,the physiological characteristics,including endogenous hormone,antioxidant enzyme,soluble protein and malondialdehyde of rice seedling were significantly(P<0.05)influenced by the supplemental light sources.Among all supplemental light sources,RBG 12.5 significantly(P<0.05)increased the content of auxin(IAA)and soluble protein,and B significantly(P<0.05)increased the content of superoxide dismutase(SOD),peroxidase(POD)and malondialdehyde(MDA)in the aboveground parts.Compared with CK,RBG 12.5 and RBG 25 significantly(P<0.05)increased the content of IAA,and RBG 25 significantly(P<0.05)increased the content of soluble protein and MDA in the belowground parts.The effect of supplemental light sources on rice yield was consistent with that on rice strong seedling index,which was decreased in the order of RBG 12.5>RBG 25>RB>B>R>CK.Stepwise regression analysis showed that strong seedling index was positively related to the content of gibberellic acid(GA 3)and IAA in the aboveground parts and the content of SOD and IAA in the belowground parts.Rice yield was positively related to the content of IAA in the aboveground parts and the content of IAA and catalase(CAT)in the belowground parts.In general,the responses of rice seedlings to LED supplementary lights were regulated by the changes in physiological characteristics,which can extend to the rice maturity period,leading to corresponding changes in the grain yield.Among all supplemental light sources,RBG 12.5 was the most suitable supplemental light for rice factory seedling nursing.
作者
刘萍
张粟
蒋世翠
黄丹丹
李成宇
张士秀
LIU Ping;ZHANG Su;JIANG Shicui;HUANG Dandan;LI Chengyu;ZHANG Shixiu(Key Laboratory of Mollisols Agroecology,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Changchun 130102,China;State Key Laboratory of Rare Earth Resources Utilization,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China;Yanbian University,Yanji 133002,China)
出处
《土壤与作物》
2021年第1期67-78,共12页
Soils and Crops
基金
吉林省优秀青年人才基金项目(20180520047JH)
长白山生物资源与功能分子教育部重点实验室开放课题(延边大学,NRFM201602)。
关键词
LED光源
水稻
秧苗素质
内源激素
抗氧化酶
壮苗指数
产量
light-emitting diodes
rice
seedling quality
endogenous hormones
antioxidant enzymes
strong seedling index
grain yield
作者简介
第一作者简介:刘萍(1986-),女,实验师,硕士,主要从事农田生态系统的研究.E-mail:415206528@qq.com;通信作者:张士秀(1986-),女,副研究员,博士,硕士生导师,主要从事农业生态系统可持续发展的基础和应用研究.E-mail:zhangshixiu@iga.ac.cn.
