摘要
目的研究大剂量五味子配伍甘草后对肝细胞脂质堆积的干预作用,并探讨相关机制。方法 SD大鼠给予五味子(SF,五味子生药量为3.9 g·kg-1的醇提物)、五味子-甘草(SG,1∶1,1∶1.5,3.9 g·kg-1),1次/d,连续给药7 d后制备含药血清。基于L02细胞,分对照组(C)、SF、SG(1∶1)和(1∶1.5)组,作用48 h后,检测LDH释放量、细胞内TG、TC含量;RT-PCR法检测PPAR-α、PPAR-γ、Fabp1/2、SREBP1c、ACCα、FAS的mRNA表达。结果与C组比,SF升高TG、TC含量,降低PPAR-α、PPAR-γ的mRNA表达,升高SREBP1c、ACCα的mRNA表达;与SF比,SG(1∶1)降低TG、TC含量,降低Fabp1/2、FAS的mRNA表达,升高SREBP1c的mRNA表达;SG(1∶1.5)仅降低TC含量,升高PPAR-γ、SREBP1c的mRNA表达,降低Fabp1/2、FAS的mRNA表达。结论五味子含药血清可显著升高肝细胞中TG、TC含量,配伍甘草可降低其升高的TG、TC含量,降低脂质堆积,其机制可能与调控PPAR-α、PPAR-γ、Fabp1/2、SREBP1c、ACCα、FAS基因mRNA的表达有关。
Aim To study the effect of drug-containing serum of Schisandra Chinensis Fructus and compatible with Glycyrrhizae Radix Et Rhizoma-on lipid accumulation in hepatocytes and explore the related mechanism.Methods SD rats were given Schisandra Chinensis Fructus(SF,3.9 g·kg^-1),Schisandrae Chinensis Fructus-Glycyrrhizae Radix Et Rhizoma(SG,1∶1,1∶1.5,the extract 3.9 g·kg^-1 in crud of Schisandrae Chinensis Fructus),once per day,the drug-containing serum was prepared after seven days of continuous administration.Conventional cultivation of human normal hepatocytes(L02 cells)in vitro,cells were divided into blank control group,SF group,and SG(1∶1 and 1∶1.5)group.After 48 hours’treatment,lactate dehydrogenase(LDH)release was detected by the kit,the levels of intracellular triglyceride(TG)and total cholesterol(TC)were detected by biochemical method.The mRNA expression levels of PPAR-α,PPAR-γ,Fabp1/2,SREBP1c,ACCαand FAS were detected by the real-time reverse tran scription polymerase chain reaction(RT-PCR).Results The biochemical results showed that compared with the blank group,the content of TG and TC in SF group increased significantly(P<0.05),the mRNA expression of PPAR-αand PPAR-γin SF group was significantly reduced,and the mRNA expression of SREBP1c and ACCαmarkedly increased(P<0.05,P<0.01).When compared with SF group,the levels of TG and TC in SG(1∶1)group were significantly reduced(P<0.05),the mRNA expressions of Fabp1/2 and FAS in SG(1∶1)group were significantly reduced,while the mRNA expression of SREBP1c significantly increased(P<0.05,P<0.01).TC content in SG(1∶1.5)group significantly decreased(P<0.05)and the mRNA expression of PPAR-γ,SREBP1c in SG(1∶1.5)significantly increased,but the Fabp1/2 and FAS markedly decreased(P<0.05,P<0.01).Conclusions SF containing serum can significantly increase the content of TG and TC in hepatocytes,and the SG containing serum can significantly improve the elevated TG and TC contents and reduce lipid accumulation.The mechanism may be related to the regulation of mRNA expression of PPAR-α,PPAR-γ,Fabp1/2,SREBP1c,ACCαand FAS.
作者
贾文汇
李伟霞
张辉
张明亮
牛璐
王炎
泥文娟
王晓艳
唐进法
JIA Wen-hui;LI Wei-xia;ZHANG Hui;ZHANG Ming-liang;NIU Lu;WANG Yan;NI Wen-juan;WANG Xiao-yan;TANG Jin-fa(School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450008, China;Henan Province Engineering Lab for Clinical Evaluation Technology of Chinese Medicine, the First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450000, China)
出处
《中国药理学通报》
CAS
CSCD
北大核心
2021年第2期276-281,共6页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 81803702)
河南省中医药拔尖人才培养项目
河南省高等学校重点科研项目(19A360007)
河南中医药大学研究生科研创新类项目(No 2019KYCX038)。
关键词
五味子
甘草
配伍
含药血清
脂质堆积
机制研究
Schisandra Chinensis Fructus
Glycyrrhizae Radix Et Rhizoma
compatibility
drug-containing serum
lipid accumulation
mechanism research
作者简介
贾文汇(1995-),女,硕士生,研究方向:中药合理用药研究,E-mail:wenhui_jia222@126.com;通讯作者:王晓艳(1988-),女,博士,主管药师,研究方向:中药临床合理用药与脂质代谢,E-mail:xiaoyan_wang1988@126.com;通讯作者:唐进法(1974-),男,博士,主任药师,研究方向:中药质量评价与临床合理用药,E-mail:a0519@163.com。
