摘要
以大豆异黄酮糖苷为原料,酶解制备苷元型大豆异黄酮。以水解率和苷元得率为指标对几种来源的β-葡萄糖苷酶、β-半乳糖苷酶、纤维素酶进行筛选,确定最适酶解用酶。通过单因素实验对酶添加量、底物质量浓度、酶解温度、pH、酶解时间进行优化。结果表明,最佳酶解工艺条件为:采用β-葡萄糖苷酶(300 U/g),酶添加量7%,底物质量浓度1.6 mg/mL,酶解温度56℃,pH 4.8,酶解时间6 h。在最佳工艺条件下,大豆异黄酮糖苷的水解率及苷元得率分别达到96.84%和99.74%。
Using soybean isoflavone glycosides as raw material,the isoflavone aglycones were prepared by enzymatic hydrolysis.With the hydrolysis rate and aglycones yield as the evaluation indexes,theβ-glucosidase,β-galactosidase and cellulase from different sources were screened to determine the optimal hydrolysis enzyme.The enzyme dosage,substrate mass concentration,enzymolysis temperature,pH and enzymolysis time were optimized by single factor experiment.The results showed that the optimal enzymolysis conditions were obtained as follows:β-glucosidase(300 U/g),enzyme dosage 7%,substrate mass concentration 1.6 mg/mL,enzymolysis temperature 56℃,pH 4.8,and enzymolysis time 6 h.Under these conditions,the hydrolysis rate of soybean isoflavone glycosides and aglycones yield reached 96.84%and 99.74%,respectively.
作者
周文红
郭咪咪
毕艳红
王朝宇
段章群
ZHOU Wenhong;GUO Mimi;BI Yanhong;WANG Zhaoyu;DUAN Zhangqun(Institute of Cereal&Oil Science and Technology,Academy of National Food and Strategic Reserves Administration,Beijing 102209,China;College of Life Sciences and Food Engineering,Huaiyin Institute of Technology,Huai’an 223003,Jiangsu,China)
出处
《中国油脂》
CAS
CSCD
北大核心
2020年第12期100-104,共5页
China Oils and Fats
基金
中央级公益性科研院所基本科研业务费专项基金(ZX1905)。
关键词
大豆异黄酮
糖苷
酶解
苷元
soybean isoflavone
glycoside
enzymatic hydrolysis
aglycone
作者简介
周文红(1993),女,硕士研究生,研究方向为生物化工(E-mail)zwh465@126.com。;通信作者:段章群,副研究员(E-mail)dzq@chinagrain.org。
