摘要
为了研究正常卵巢和囊肿卵巢中脂类代谢相关基因FABP3、FABP4、CPT1的表达,分别收集卵巢组织、颗粒细胞、卵母细胞,运用实时荧光定量PCR技术对上述3个基因的表达进行比较。结果显示:囊肿卵巢组织中FABP3和CPT1表达水平显著高于正常卵巢组织,囊肿卵巢组颗粒细胞中FABP3表达水平极显著高于正常卵巢组;囊肿卵巢卵母细胞中CPT1、FABP4的表达极显著高于正常卵巢GV期和MⅡ期卵母细胞。本研究可为深入研究脂质代谢基因在猪卵巢囊肿发生中的作用提供依据。
In order to investigate the expression of lipid metabolism genes FABP3、FABP4、CPT1 involved in porcine normal and cyst ovary, ovarian tissues, granulosa cells and oocytes were collected, and the expression of genes were detected by real-time fluorescence quantitative PCR. The results showed that the expressions of FABP3 and CPT1 in porcine cyst tissues were significantly higher than those in normal ovarian tissues, the expression of FABP3 in granulosa cells of cyst ovary was significantly higher than that of normal ovary, and the expression of CPT1 and FABP4 in porcine cyst oocyte were significantly higher than that in normal ovarian GV and MⅡ oocyte. This research would provide a basis for further study of the role of lipid metabolism genes in the development of porcine ovarian cysts.
作者
罗晓彤
吕香州
李欣
张琪
于永生
田子玉
金一
LUO Xiaotong;LYU Xiangzhou;LI Xin;ZHANG Qi;YU Yongsheng;TIAN Ziyu;JIN Yi(College of Agronomy,Yanbian University,Yanji 133002;Jilin Academy of Agricultural Sciences,Changchun 130033,China)
出处
《东北农业科学》
北大核心
2020年第5期62-64,144,共4页
Journal of Northeast Agricultural Sciences
基金
国家自然科学基金(31960659)
吉林省科技厅重点科技攻关项目(20190301037NY)。
关键词
猪
囊肿卵巢
颗粒细胞
卵母细胞
脂质代谢
Susscrofa domestica
Cyst ovary
Granulosa cell
Oocyte
Lipid metabolism
作者简介
罗晓彤(1981-),女,在读博士,主要从事动物繁殖与生物技术研究;通讯作者:田子玉,男,硕士,副研究员,E-mail:tzy19720220@126.com;金一,男,博士,教授,E-mail:yijin@yhu.edu.cn。
