摘要
为探究绿原酸对猪精液冷冻保存效果的影响,分别在TCG稀释液中添加不同浓度(15、30、50、80和100μg/mL)的绿原酸,通过测定冷冻-解冻后精子的活率、顶体完整率、质膜完整率、DNA完整率、超氧化歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活性来判定对保存效果的影响。结果表明,当添加剂量为50μg/mL时,保护效果最好,精子的活率、顶体完整率、质膜完整率、SOD、CAT和GSH-Px活性等参数均高于对照组(P<0.05);在不同处理组中,冻后精子活率和SOD活性在添加剂量为30和50μg/mL时,较其他处理组差异显著(P<0.05);质膜完整率、CAT和GSH-Px活性在添加剂量为15和50μg/mL时,较其他处理组差异显著(P<0.05)。试验结果表明,TCG稀释液中添加50μg/mL绿原酸对猪精液冷冻保存效果最佳。
To explore the effect of chlorogenic acid on the cryopreservation of boar semen,different concentrations(15,30,50,80 and 100μg/ml)of CGA were added to the TCG diluent respectively.The quality of spermatozoa was determined by measuring the activity rate,acrosome integrity rate,plasma membrane integrity,DNA integrity,superoxide dismutase,catalase and glutathione peroxidase activity after freezing-thawing.The results showed that when the concentration of additives was 50μg/ml,the protection effect was the best,the plasma membrane integrity rate,SOD,CAT and GSH-Px activities were significantly higher than those of the control group(P<0.05);in different treatment groups,when the addition dose was 30 and 50μg/ml,the difference was significant compared with other treatment groups(P<0.05);when the addition dose was 15 and 50μg/ml,the difference was significant compared with other treatment groups(P<0.05).The results showed that adding 50μg/ml CGA had the best effect on cryopreservation of boar semen.
作者
何涛
李青旺
余四九
HE Tao;LI Qingwang;YU Sijiu(Gansu Agricultural University,Lanzhou Gansu 730070,China;Ningxia Polytechnic,Yinchuan Niangxia 750021,China;Northwest A&F University,Yangling Shaanxi 712100,China)
出处
《家畜生态学报》
北大核心
2021年第1期56-60,共5页
Journal of Domestic Animal Ecology
关键词
绿原酸
猪精液
冷冻保存
精子质量
chlorogenic acid
boar sperm
cryopreservation
sperm quality
作者简介
何涛(1975-),男,宁夏中宁人,博士,研究方向:畜禽繁殖与育种。E-mail:Hetao0001@163.com;通讯作者:余四九(1961-),男,甘肃秦安人,博士生导师,研究方向:动物胚胎工程。E-mail:yusj@gsau.edu.cn。
