摘要
目的建立朝药材肾炎草的HPLC-UV指纹图谱。方法采用SunFire^(TM) C_(18)(4.6 mm×250 mm, 5μm)色谱柱;以乙腈(B)-0.4%冰醋酸溶液(A)梯度洗脱,检测波长253 nm,柱温40℃,进样量10μL,流速1.0 mL·min^(-1),采用对照品指认进行成分分析。结果建立了朝药材肾炎草的HPLC-UV特征指纹图谱共有模式,标定了21个共有峰,指认了其中3个黄酮碳苷成分。10批次药材的相似度结果均大于0.95,相似程度高。结论该方法简便,精密度、重复性和稳定性很好,特征性和专属性强,可为肾炎草药材整体质量控制提供有效手段。
OBJECTIVE To establish the fingerprint of Melandrium firmum Rohrb.by HPLC-UV.METHODS The analysis was performed on SunFireTM C18(4.6 mm×250 mm,5μm)column.The mobile phase was acetonitrile(B)and 0.4%phosphoric acid(A)with gradient elution.The detection wavelength and column temperature were 253 nm and 40℃,respectively.The flow rate was 1.0 mL·min-1.The injection volume was 10μL.The chemical attribution of the fingerprint was determined by reference substance comparison method.RESULTS The HPLC-UV fingerprints of 10 batches of M.firmum were calibrated with 21 common peaks.Among them,three flavone C-glycosides were identified.The similarities of the chromatograms of 10 batches of M.firmum were greater than 0.95.CONCLUSION The method is simple and reliable,which has satisfactory precision,repeatability and stability.It can be used for the quality control of M.firmum.
作者
蔺海灵
程盛宇
李娜
Alaa Elshafei
于跃
郑明善
LIN Hai-ling;CHENG Sheng-yu;LI Na;Alaa Elshafei;YU Yue;ZHENG Ming-shan(School of Pharmaceutical Sciences,Yanbian University,Yanji 133002,China)
出处
《中国药学杂志》
CAS
CSCD
北大核心
2020年第22期1847-1852,共6页
Chinese Pharmaceutical Journal
基金
国家自然科学基金地区项目资助(81660646)
吉林省中医药管理局科技项目资助(2015ZT52)。
关键词
朝药材
肾炎草
高效液相色谱法
指纹图谱
黄酮碳苷
Korean Chinese folk medicine
Melandrium firmum Rohrb.
HPLC
fingerprint
flavone C-glycoside
作者简介
蔺海灵,女,硕士研究方向:中药材质量标准研究;通讯作者:郑明善,女,副教授,硕士生导师研究方向:中药药效物质基础与中药材质量标准研究,Tel:(0433)2436016,E-mail:zhengmingshan@ybu.edu.cn。
