摘要
为提高箬叶的药用价值,本文通过高效液相色谱(HPLC)法分析荭草苷、异荭草苷、牡荆苷、异牡荆苷等箬叶中主要黄酮碳苷的含量,采用梯度洗脱法,考察了聚酰胺树脂分离纯化这4种黄酮碳苷的效果。结果表明:采用30～60目聚酰胺树脂,经乙醇-水初步梯度洗脱后,4种黄酮碳苷在30%和50%乙醇洗脱剂中基本洗脱完全;采用80～100目聚酰胺树脂,经乙酸乙酯-乙醇-水的进一步梯度洗脱后,总黄酮的含量为94.69%,纯化倍数为16.3;4种黄酮碳苷纯化倍数超过25倍,回收率达91%以上。研究结果可为箬叶黄酮碳苷的进一步利用提供参考。
In order to impro ve the medicinal value of indocalamus leaves, the main c-glycosylflavones of indocalamus leaves such as orientin, isoorientin, vitexin, isovitexin were determined by HPLC, the separation and purification of the four c-glycosylflavones from indocalamus leaves using polyamide resin coupled with gradient elution were studied. The experimental results showed that:(1) four kinds of flavonoid glycosides could be eluted completely by 30% and 50% ethanol elution agent after preliminary gradient elution of ethanol-water,when using polyamide resin with 30 -60 mesh;(2) the content of total flavonoids was 94.69% and the purification fold was16.3 after further gradient elution of ethyl acetate-ethanol-water,when using polyamide resin with 80-100 mesh,the purification fold of four kinds of flavonoids glycosides was more than 25 times, the recovery rate was larger than 91%. Which would provide a reference for the further utilization of c-glycosylflavones in indocalamus leaves.
作者
乐薇
赵淳
韦琴
YUE Wei;ZHAO Chun;WEI Qin(College of Environmental and Biological Engineering,Wuhan Technology and Business University, Wuhan 430065,China)
出处
《保鲜与加工》
CAS
北大核心
2018年第6期100-106,共7页
Storage and Process
基金
湖北省教育厅科研项目(B2017321)
武汉工商学院校学术团队资助项目(XSTD2015003)
关键词
箬叶
黄酮碳苷
聚酰胺树脂
梯度洗脱
indocalamus leaf
C-glycosylflavones
polyamide resin
gradient elution
作者简介
乐薇(1979-),女,汉族,硕士,副教授,主要从事生物分析研究工作。
