摘要
以自然衰老凋落的枇杷Eriobotrya japonica叶片为原料,以水、乙醇、乙酸乙酯、正丁醇为溶剂,采用索式抽提,研究其提取物中多酚、总糖、花青素、黄酮类化合物的含量和得率,并分析其抗氧化能力。结果表明:4种溶剂中混合天然产物提取得率最高的为水提,其得率为22.1%;提取物中多酚得率和含量最高的溶剂分别为水和乙醇,分别为9.5%和0.509 mg·mg^-1;提取物中总糖提取得率和含量最高的溶剂均为水,分别为4.97%和0.225 mg·mg^-1;乙醇对花青素的提取得率最高为1.08%,提取物中含量最高的为乙酸乙酯,为0.113 mg·mg^-1;提取物中黄酮类化合物提取得率和含量最高的均为乙醇,分别为3.04%和0.210 mg·mg^-1;抗氧化能力最强的为乙醇提取物,为0.446μmol×mg^-1。结论:自然衰老的枇杷叶具有较高的开发价值和广阔的应用前景,可依据总糖和总酚等不同组分提取目标选择适宜的溶剂体系。
In February and March 2019,fallen leaves were collected in Eriobotrya japonica orchard in Hangzhou,Zhejiang province.Experiments were conducted on extraction by Sowhlet extractor method with different solvents such as water,ethanol,ethyl acetate and n-butanol.The results demonstrated that the highest yield of mixed natural product was by water,topped to 22.1%.The highest extraction rate and content of polyphenols was extracted by water and ethanol respectively,9.5%and 0.509 mg/mg.The highest total carbohydrate rate and extract content was by water,4.97%and 0.225 mg/mg.The highest extraction rate of anthocyanin was 1.08%in ethanol,and the highest extracted content was 0.113 mg/mg in ethyl acetate.The highest extraction rate and content of total flavonoids was 3.04%and 0.210 mg/mg in ethanol.Analysis on antioxidant capacity showed that the best was ethanol extract,0.446μmol/mg.
作者
潘炘
庄晓伟
于海霞
蒋应梯
张文福
王进
PAN Xin;ZHUANG Xiao-wei;YU Hai-xia;JIANG Ying-ti;ZHANG Wen-fu;WANG Jin(Zhejiang Academy of Forestry,Zhejiang Provincial Key Laboratory of Biological and Chemical Utilization of Forest Resources,Hangzhou 310023,China)
出处
《浙江林业科技》
2020年第6期18-23,共6页
Journal of Zhejiang Forestry Science and Technology
基金
浙江省重点研发项目(2017C02042)
浙江省科研院所扶持专项(2019F1065)。
关键词
枇杷落叶
提取物
多酚化合物
黄酮类化合物
总糖
花青素
leaf of Eriobotrya japonica
extraction
polyphenols
flavonoids
total carbohydrate
anthocyanin
作者简介
潘炘,副研究员,从事林产化学与天然产物研究工作;E-mail:panxin@sohu.com;通信作者:庄晓伟,副研究员,从事林产化工究工作;E-mail:Zhuang413@sina.com。
