摘要
目的评估支原体RNA实时荧光恒温扩增技术(MP-SAT)在儿童支原体肺炎抗生素治疗中的指导价值。方法选取成都儿童专科医院2019年1-12月因支原体肺炎住院且咽拭子MP-SAT检测阳性的患儿70例为研究对象。入院当天,在使用抗生素治疗前,采集患儿咽拭子。予阿奇霉素抗感染治疗2~4周,MP-SAT结果转阴后,停用阿奇霉素治疗。在治疗开始后,每周复查MP-SAT及胸部X线片各1次(每种检查结果转阴后不再复查),分别记录两种检查结果及临床症状体征的恢复情况。结果MP-SAT转阴率随抗生素治疗时间延长而增高,MP-SAT转阴时间(2.41±0.62)周;胸部X线片转阴率随治疗时间延长而增高,胸部X线片转阴时间(3.40±1.46)周;体温恢复正常时间为(1.14±0.35)周,其他临床症状体征恢复较慢,临床症状体征恢复正常时间为(3.26±1.16)周;MP-SAT转阴时间与胸部X线片转阴时间比较,差异有统计学意义(t=5.65,P<0.001);MP-SAT转阴时间与临床症状体征恢复正常时间比较,差异有统计学意义(t=4.15,P<0.001)。结论在儿童支原体肺炎抗生素治疗过程中,MP-SAT转阴时间短于胸部X线片转阴时间和临床症状体征恢复正常时间。在评估支原体肺炎疗效指标中,使用MP-SAT转阴指标优于传统胸部X线片和临床症状体征恢复正常的指标。
Objective To evaluate the guiding value of mycoplasma RNA real-time fluorescence thermostatic amplification(MP-SAT)in antimicrobial therapy for mycoplasma pneumonia in children.Methods From January 2019 to December 2019,a total of 70 children with positive MP-SAT throat swabs who were hospitalized for mycoplasma pneumonia in Chengdu Children's Specialized Hospital were selected as study subjects.On the day of admission,throat swabs were collected before antimicrobial therapy.Azithromycin was used for anti infection treatment for 2 to 4 weeks.And when MP-SAT results turned negative,azithromycin treatment was stopped.Since the beginning of treatment,MP-SAT and chest X-ray were reviewed once a week(no reexamination was required after each examination result turned negative).The results of the two examinations and the recovery of clinical symptoms and signs were recorded respectively.Results The negative conversion ratio of MP-SAT increased with the prolongation of antimicrobial therapy.And the time required for MP-SAT to turn negative was(2.41±0.62)weeks.The negative conversion ratio of chest X-ray increased with the extension of antimicrobial therapy.And the time required for chest X-ray to turn negative was(3.40±1.46)weeks.The time for body temperature to return to normal was(1.14±0.35)weeks.The recovery of other clinical symptoms and signs was slow.And the time needed for clinical symptoms and signs to return to normal was(3.26±1.16)weeks.The difference was statistically significant when comparing the time needed for MP-SAT to turn negative with that needed for chest X-ray to turn negative(t=5.65,P<0.001).The difference was statistically significant between the time required for MP-SAT to turn negative and that for clinical symptoms and signs to return to normal(t=4.15,P<0.001).Conclusion In the course of antimicrobial therapy for mycoplasma pneumonia in children,the time required for MP-SAT to turn negative was shorter than that for chest X-ray to turn negative and that for clinical symptoms and signs to return to normal.In the evaluation of the efficacy indicators of mycoplasma pneumonia,the MP-SAT negative index was superior to the traditional indexes of chest X-ray and normal clinical symptoms and signs.
作者
王紫荆
汪蓉
胡小林
方剑
赵云
尹清金
侯晓华
谢屹峰
朱静
马云驰
庄琴
Wang Zijing;Wang Rong;Hu Xiaolin;Fang Jian;Zhao Yun;Yin Qingjin;Hou Xiaohua;Xie Yifeng;Zhu Jing;Ma Yunchi;Zhuang Qin(Ward 1, Department of Internal Medicine, Chengdu Children's Specialized Hospital, Chengdu 610015, China;Department of Pediatrics, The Second Affiliated Hospital of Chengdu Medical College·416 Hospital of Nuclear Industry, Chengdu 610051, China)
出处
《成都医学院学报》
CAS
2020年第6期716-719,共4页
Journal of Chengdu Medical College
基金
四川省卫生和计划生育委员会科研项目(No:17PJ267)。
关键词
RNA实时荧光恒温扩增技术
支原体肺炎
抗生素
疗效
Real-time fluorescence thermostatic amplification of RNA
Mycoplasma pneumonia
Antimicrobial agents
Curative effect
作者简介
通讯作者:汪蓉,E-mail:96553053@qq.com。
