摘要
建立了高效液相色谱法测定饲料中甲硝唑(MNZ)、替硝唑(TNZ)、地美硝唑(DMZ)、奥硝唑(ONZ)和异丙硝唑(IPZ)等5种硝基咪唑类药物的方法。样品用乙酸乙酯提取,正己烷-磷酸溶液反萃取,MCX柱净化,采用Agilent Eclipse Plus C18色谱柱(4.6 mm×250 mm,5.0μm)分离五种硝基咪唑类药物,以甲醇和水为流动相进行梯度洗脱,流速0.8 ml/min,采集波长范围为200~400 nm,记录光谱图和320 nm波长处的色谱图。结果显示,五种硝基咪唑类药物的浓度在0.1~25μg/ml范围内的线性良好,平均加标回收率在70.8%~92.1%范围内,RSD(n=5)在5.6%~10.8%之间,检测限均为0.1 mg/kg。本方法快速、准确,可用于饲料中非法添加5种硝基咪唑类药物的定性和定量检测。
A HPLC-DAD method for the determination of metronidazole,tinidazole,dimetridazole,orni⁃dazole and ipronidazole in feed was established.Samples were extracted with ethyl acetate,and puri⁃fied by hexane-phosphoric acid solution.Then the acidic solution was applied to MCX cartridge for further cleanup.The Agilent Eclipse Plus C18 column(4.6 mm×250 mm,5.0μm)was used to sepa⁃rate five nitroimidazals.Gradient elution was conducted with methanol and water as mobile phases at a flow rate of 0.8 ml/min.The scanning range was within 200~400 nm,and the detection wavelength was at 320 nm.The linearity of the five nitroimidazole drugs was good in the range of 0.1~25μg/ml.The average recovery rates were from 70.8%~92.1%,the RSD(n=5)was within the range of 5.6%~10.8%,and the detection limit was all 0.1 mg/kg.This method is rapid and accurate,and can be used for the qualitative and quantitative detection of five kinds of nitroimidazoles in feed.
作者
罗成江
张晓丽
陈晓琳
林仙军
王彬
阮鑫
Luo Chengjiang;Zhang Xiaoli;Chen Xiaolin;Lin Xianjun;Wang Bin;Ruan Xin
出处
《饲料工业》
北大核心
2020年第22期52-56,共5页
Feed Industry
基金
浙江省重点研发计划项目“新型兽药研制及耐药性控制技术研发”[2020C02032]。
作者简介
罗成江,兽医师,研究方向为兽药、饲料、畜产品安全检测技术。
