摘要
该研究基于网络药理学方法探讨"半枝莲-白花蛇舌草"抗乳腺癌的作用机制。通过中药系统药理学分析平台(TCMSP)筛选半枝莲、白花蛇舌草的有效成分及相应的靶蛋白,通过Genecards,OMIM数据库筛选出乳腺癌的靶点,利用Venn在线软件获取药物与疾病的共同靶点,运用Cytoscape 3.7.2软件构建"化合物-靶点-疾病"网络图。使用STRING数据库绘制蛋白互作(PPI)网络,利用David数据库对有效作用靶点进行GO功能、KEGG通路富集分析。结果显示,共筛选出半枝莲化合物29个,白花蛇舌草化合物7个,有效作用靶点109个。通过Venn图获得59个药物疾病共同靶点,PPI网络发现MYC,CCND1,EGFR,ESR1,CASP3,VEGFA等可能是"半枝莲-白花蛇舌草"治疗乳腺癌的关键靶点。GO功能分析发现88个条目,涉及转录因子活性、受体活性、细胞因子活性、酶活性及生物合成过程的调控等方面。KEGG通路分析发现37个条目,涉及到癌症、细胞周期、细胞凋亡、血管生成等通路,其中包括p53信号通路、ErbB信号通路、NOD样受体信号通路、MAPK信号通路、VEGF信号通路、Wnt信号通路、mTOR信号通路等。该研究初步揭示了"半枝莲-白花蛇舌草"抗乳腺癌的关键靶点和涉及的生物学过程及信号通路,发现其作用是多靶点、多通路的,为今后的分子生物学实验奠定了基础。
To explore the mechanism of Scutellaria barbata-Hedyotis diffusa against breast cancer based on network pharmacological methods.The active components and corresponding target proteins of S.barbata and H.diffusa were screened by using Traditional Chinese Medicine Systems Pharmacology Database(TCMSP),and the targets of breast cancer were screened through the Genecards and OMIM databases.Venn online software was used to obtain the common targets of drugs and the disease,and then the"compound-target-disease"network diagram was constructed by using Cytoscape 3.7.2 software.The STRING database was used to draw the protein-protein interaction(PPI)network,and the David database was used to perform GO function and KEGG pathway enrichment analysis on effective targets.The results showed that 29 S.barbata compounds,7 H.diffusa compounds,and 109 effective targets were screened.The 59 common targets for drugs and disease were obtained through Venn diagrams.The PPI network showed that MYC,CCND1,EGFR,ESR1,CASP3,VEGFA,etc.might be the key targets for"S.barbata-H.diffusa"in the treatment of breast cancer.In the GO function analysis,88 entries were found,involving the regulation of transcription factor activity,receptor activity,cytokine activity,enzyme activity,and biosynthetic processes.In KEGG pathway analysis,37 entries related to cancer,cell cycle,apoptosis,angioge-nesis and other pathways were found,including p53 signaling pathway,ErbB signaling pathway,nod like receptor signaling pathway,MAPK signaling pathway,VEGF signaling pathway,Wnt signaling pathway and mTOR signaling pathway and other classic signaling pathways.This study preliminarily revealed the key targets,biological processes and signal pathway of"S.barbata-H.diffusa"against breast cancer.It was found that its function was in a multi-target and multi-channel manner,which laid a foundation for future molecular biological experiments.
作者
许晶
石凤芹
杜可心
董佳
李冬云
XU Jing;SHI Feng-qin;DU Ke-xin;DONG Jia;LI Dong-yun(Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2020年第18期4448-4454,共7页
China Journal of Chinese Materia Medica
基金
国家自然科学基金青年基金项目(81603583)。
关键词
半枝莲-白花蛇舌草
乳腺癌
网络药理学
作用机制
Scutellaria barbata-Hedyotis diffusa
breast cancer
network pharmacology
mechanism of action
作者简介
许晶,硕士研究生,E-mail:8515217I5@qq.com;通信作者:李冬云,医学博士,教授,主任医师,主要从事中西医结合防治血液病、恶性肿瘤的临床、科研及教学,E-mail:lidyOO39@sina.com。
