摘要
目的:探讨人β-防御素-3(hBD-3)在口腔黏膜下纤维性变(OSF)发生及癌变过程中的表达变化及其意义。方法:采用免疫组织化学以及实时荧光定量PCR技术检测10例正常口腔黏膜、22例OSF、13例伴OSF口腔鳞癌组织中hBD-3表达水平。结果:hBD-3蛋白在正常口腔黏膜、OSF、伴OSF口腔鳞癌组织中阳性表达率为40.00%、86.36%、100.00%(P<0.05)。正常口腔黏膜、OSF、伴OSF口腔鳞癌组织中hBD-3 mRNA相对表达量分别为1.06±0.37、1.73±0.21、7.07±0.88(P<0.05)。结论:hBD-3可能与OSF发生及其癌变有关。
Objective:To investigate the expression and significance of humanβ-defensin-3(hBD-3)in the progress from normal mucosa to oral submucosal fibrosis(OSF)and to carcinogenesis.Methods:Immunohistochemistry and real-time fluorescent quantitative PCR technique were used to detect the expression of hBD-3 in 10 cases of normal oral mucosa,22 cases of OSF and 13 cases of oral squamous cell carcinoma with OSF.Results:The positive rate of hBD-3 protein in normal oral mucosa tissues,OSF tissue,oral squamous cell carcinoma with OSF tissue was 40.00%,86.36%and 100.00%(P<0.05)respectively.The relative expression of hBD-3 mRNA in normal oral mucosa tissues,OSF tissue and oral squamous cell carcinoma with OSF tissue was 1.06±0.37,1.73±0.21,7.07±0.88(P<0.05)respectively.Conclusion:hBD-3 may be related to the occurrence and carcinogenesis of OSF.
作者
杨琴
杜永秀
翦新春
杨晨希
徐普
YANG Qin;DU Yongxiu;JIAN Xinchun;YANG Chenxi;XU Pu(Department of Oral Mucosal Diseases,Stomatology Center,Affiliated Haikou Hospital of Xiangya Medical School,Center South University,570208 Haikou,China;Department of Oral and Maxillofacial Surgery,Xiangya Hospital,Central South University,Changsha)
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2020年第5期795-798,共4页
Journal of Practical Stomatology
基金
海南省自然科学基金(编号:817381)
海口市人民医院院内课题(编号:2017-YNK-10)。
作者简介
通信作者:徐普,E-mail:hnxupu@163.com。
