摘要
本试验旨在研究饲粮中添加不同水平豌豆和复合酶制剂对肉仔鸡生长性能、养分利用及肠道发育的影响。采用5×2析因试验设计,A因子为豌豆添加水平(A1:0、A2:10%、A3:20%、A4:30%、A5:40%),B因子为复合酶制剂添加水平(B1:0 U/kg淀粉酶和0 U/kg蛋白酶、B2:750 U/kg淀粉酶和60 U/kg蛋白酶)。选择体重相近的1日龄爱拔益加(AA)肉仔鸡960只,随机分为10个组,分别为A1B1组、A1B2组、A2B1组、A2B2组、A3B1组、A3B2组、A4B1组、A4B2组、A5B1组和A5B2组,每组8个重复,每个重复12只鸡(公母各占1/2)。试验期42 d。结果表明:1)豌豆添加水平对肉仔鸡的平均日采食量(ADFI)无显著影响(P>0.05),对平均日增重(ADG)和料重比(F/G)有显著影响(P<0.05);饲粮中添加10%和20%豌豆对F/G无显著影响(P>0.05),当豌豆添加水平为30%和40%时ADG显著降低(P<0.05),F/G显著增加(P<0.05)。复合酶制剂添加水平及豌豆和复合酶制剂添加水平的交互作用对肉仔鸡的生长性能均无显著影响(P>0.05)。2)豌豆添加水平对肉仔鸡的干物质、粗蛋白质、能量、钙和磷利用率有显著影响(P<0.05);饲粮中添加10%和20%豌豆对能量和磷利用率无显著影响(P>0.05)。复合酶制剂添加水平对肉仔鸡的养分利用率无显著影响(P>0.05);豌豆和复合酶制剂添加水平对粗蛋白质、钙和磷利用率有显著交互作用(P<0.05)。3)饲粮中添加40%豌豆显著增加肉仔鸡的空肠长度(P<0.05),750 U/kg淀粉酶和60 U/kg蛋白酶复合酶制剂添加水平显著增加盲肠重量(P<0.05),有提高盲肠质量指数的趋势(0.05≤P<0.10);豌豆和复合酶制剂添加水平对肠道发育无显著交互作用(P>0.05)。综上所述,饲粮中添加10%和20%豌豆对肉仔鸡的生长性能和养分利用率无不利影响,并可促进空肠及全肠道发育;饲粮中添加30%和40%豌豆显著降低肉仔鸡的生长性能及养分利用率。因此可在肉仔鸡饲粮中添加不超过20%的豌豆替代玉米和豆粕。
This experiment was conducted to investigate the effects of different levels of pea and compound enzyme preparation in diets on growth performance,nutrient utilization rate and intestinal development.The 5×2 factorial test design was adopted,factor A was pea supplemental level(A1:0,A2:10%,A3:20%,A4:30%,A5:40%),and factor B was compound enzyme preparation supplemental level(B1:0 U/kg amylase and 0 U/kg protease,B2:750 U/kg amylase and 60 U/kg protease).Nine hundred and sixty one-day-old Arbor Acres(AA)broilers with similar body weight were randomly divided into 10 groups with 8 repelicates per group and 12 broilers per replicate(males and females in half),and the 10 groups were groups A1B1,A1B2,A2B1,A2B2,A3B1,A3B2,A4B1,A4B2,A5B1 and A5B2,respectively.The experiment lasted for 42 days.The results showed as follows:1)pea supplemental level had no significant effect on average daily feed intake(ADFI)of broilers(P>0.05),but had significant effects on average daily gain(ADG)and the ratio of feed to gain(F/G)(P<0.05).Diets supplemented with 10%and 20%of pea had no significant effect on F/G(P>0.05),but diets supplemented with 30%and 40%pea significantly decreased ADG and significantly increased F/G(P<0.05).Compound enzyme preparation supplemental level and the interaction of pea and compound enzyme preparation supplemental level had no significant effect on growth performance of broilers(P>0.05).2)Pea supplemental level had significant effects on the utilization rate of dry matter,crude protein,energy,calcium and phosphorus of broilers(P<0.05),and diets supplemented with 10%and 20%pea had no significant effects on the utilization rate of energy and phosphorus(P>0.05).Compound enzyme preparation supplemental level had no significant effect on nutrient utilization rate of broilers(P>0.05),and pea and compound enzyme preparation supplemental level had significant interaction on utilization rate of crude protein,calcium and phosphorus(P<0.05).3)Dietary 40%pea significantly increased jejunum length of broilers(P<0.05).Diets supplemented with 750 U/kg amylase and 60 U/kg protease compound enzyme preparation significantly increased cecum weight(P<0.05),and had a tendency of increasing cecum mass index(0.05≤P<0.10).Pea and compound enzyme preparation supplemental level had no significant interaction on intestinal development(P>0.05).In summary,diets supplemented with 10%and 20%pea have no adverse effects on growth performance and nutrient utilization rate of broilers,and promote jejunal and total intestinal development;and diets supplemented with 30%and 40%pea significantly reduce growth performance and nutrient utilization rate of broilers.So no more than 20%pea can added to broiler’s diets to replaced corn and soybean meal.
作者
刘雪
王璇
唐德富
杜宝龙
年芳
LIU Xue;WANG Xuan;TANG Defu;DU Baolong;NIAN Fang(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;College of Science,Gansu Agricultural University,Lanzhou 730070,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2020年第9期4429-4440,共12页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家自然科学基金项目(31702131,31660664)
甘肃省教育厅高等学校科研项目(2018A-041)
甘肃省现代农业产业技术体系(GARS-ZJ-3)
甘肃农业大学学科建设开放课题(GAU-XKGS-2018-07)。
关键词
豌豆
酶
肉仔鸡
生长性能
养分利用
肠道发育
pea
enzyme
broilers
growth performance
nutrient utilization rate
intestinal development
作者简介
刘雪(1994-),女,江苏徐州人,硕士研究生,从事动物营养与饲料研究。E-mail:1771375278@qq.com;通信作者:年芳,教授,硕士生导师,E-mail:nianf@gsau.edu.cn。
