摘要
采用紫外线及常压室温等离子体(ARTP)复合诱变技术对白曲霉(Aspergillus candidus)Nz 3.602进行诱变选育。对诱变致死率进行测定,确定最佳诱变条件为:15 W紫外灯照射12 min,ARTP处理40 s。对突变菌株进行透明圈法初筛、糖化力测定法复筛,最终得到产酶能力较高的突变菌株B5。其糖化酶酶活为1050.32 U/g,较出发菌株糖化酶酶活提高85.53%,并且遗传稳定性较好。以菌株B5为研究对象,经单因素试验和响应面分析法对其固态发酵条件进行优化,最佳固态发酵条件为:糠壳添加量15%,原料含水量70%,接种量12%,培养时间5 d,培养温度30℃。该条件下糖化酶酶活为(1254.17±6.14)U/g,比优化前提高了19.41%。
Aspergillus candidus Nz 3.602,a starting strain,was mutated by ultraviolet(UV)and atmospheric and room temperature plasma(ARTP).According to the lethality of mutagenesis,the optimal mutagenesis conditions were determined as follows:ultraviolet irradiation for 12 min and ARTP treatment for 40 s.The mutant strains were screened by the transparent circle method and re-screened by determining saccharification power,and finally the mutant strain B5 with higher enzyme production capacity was obtained.The saccharification enzyme activity of the strain B5 was 1050.32 U/g,which was 85.53%higher than that of the starting strain,and the genetic stability was better.The solid-state fermentation conditions of the strain B5 were optimized by single factor tests and response surface methodology,and the optimal solid-state fermentation conditions were determined as follows:bran shell 15%,moisture 70%,inoculum 12%,cultivation time 5 d and temperature 30℃.Under the optimal conditions,the saccharifying enzyme activity could reach(1254.17±6.14)U/g,which was 19.41%higher than that of before optimization.
作者
赵志军
李敏
刘延波
刘宁
葛少华
潘春梅
孙西玉
ZHAO Zhijun;LI Min;LIU Yanbo;LIU Ning;GE Shaohua;PAN Chunmei;SUN Xiyu(College of Food and Biological Engineering(Liquor College),Henan University of Animal Husbandry and Economy,Zhengzhou 450046,China;Henan Liquor Style Engineering Technology Research Center,Henan University ofAnimal Husbandry and Economy,Zhengzhou 450046,China;Baofeng Liquor Industry Co.,Ltd.,Baofeng 467500,China;Zhang-GongLao-Jiu Liquor Co.,Ltd.,Ningling 476733,China)
出处
《中国酿造》
CAS
北大核心
2020年第9期80-85,共6页
China Brewing
基金
河南省重大科技专项(181100211400)
河南牧业经济学院重点学科项目
河南省科技攻关项目(192102110215)。
关键词
糖化酶
常压室温等离子体
固态发酵
响应面法
白曲霉
glucoamylase
atmospheric room temperature plasma
solid-state fermentation
response surface method
Aspergillus candidus
作者简介
赵志军(1973-),男,讲师,本科,研究方向为食品微生物;通讯作者:孙西玉(1965-),男,教授,本科,研究方向为酿酒工程。
