摘要
为了研究硒化牡丹籽粕多糖的抗氧化活性,水提醇沉法提取牡丹籽粕粗多糖,离子交换层析制备牡丹籽粕多糖(peony seed dreg polysaccharides,PSDP)PSDPⅠ、PSDPⅡ及PSDPⅢ。硝酸-亚硒酸钠法对PSDPⅢ进行硒化修饰得Se-PSDPⅢ。采用体外抗氧化实验,评价Se-PSDPⅢ对1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基和羟自由基(·OH)的清除作用及对DNA氧化损伤的抑制作用。并以巨噬细胞RAW264.7建立H 2O 2诱导的氧化损伤细胞模型,探讨Se-PSDPⅢ在细胞水平的抗氧化能力。结果表明:Se-PSDPⅢ具有较好的体外清除自由基的能力和抑制DNA氧化损伤的能力。细胞模型法评价结果显示,Se-PSDPⅢ可以降低H 2O 2诱导的巨噬细胞RAW264.7内活性氧(reactive oxygen species,ROS)的生成。此外,Se-PSDPⅢ可以升高细胞内超氧化物歧化酶(superoxide dismutase,SOD)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)的活力。因此,硒化牡丹籽粕多糖作为潜在的抗氧化物,可用于功能产品的开发。
To investigate the antioxidant activity of selenium-containing peony seed dreg polysaccharides,the crude polysaccharides were separated by water extraction and alcohol precipitation,which was further purified by ion-exchange chromatography to obtain main polysaccharides(PSDP I,PSDP II and PSDP III).The nitric acid-sodium selenite(HNO 3-Na 2SeO 3)method was used to obtain Se-PSDP III.In vitro antioxidant experiment was used to evaluate the scavenging effect of Se-PSDPⅢon 1,1-diphenyl-2-picrylhydrazyl(DPPH)free radicals and hydroxyl free radicals(·OH)and inhibition of DNA oxidative damage.In addition,macrophage RAW264.7 was used to establish a H 2O 2-induced oxidative damage cell model to explore the antioxidant capacity of Se-PSDPⅢat the cellular level.The results demonstrated that Se-PSDP III showed a good ability to scavenge free radicals in vitro and inhibit DNA oxidative damage.The cell model evaluation results showed that Se-PSDP III could protect RAW264.7 macrophages against H 2O 2-induced oxidative damage by decreasing reactive oxygen species(ROS)production.Moreover,Se-PSDP III could increase the activity of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px).Therefore,Se-PSDP III could be used as potential antioxidants in the development of functional products.
作者
裴晋红
宋英达
武翠玲
PEI Jinhong;SONG Yingda;WU Cuiling(Changzhi Medical College,Changzhi 046000,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2020年第17期174-179,共6页
Food and Fermentation Industries
基金
山西省高等学校科技创新项目(2019L0675)
国家自然科学基金青年基金项目(31900038)。
关键词
牡丹籽粕
多糖
硒化修饰
DPPH自由基清除率
抗氧化
peony seed dreg
polysaccharide
selenium modification
DPPH scavenging capacity
antioxidant
作者简介
第一作者:裴晋红,硕士,副教授;通讯作者:武翠玲,副教授,Email:clw928@163.com。
