摘要
目的监测北京市市售扇贝中诺如病毒和A组轮状病毒污染状况,分析其基因特征。方法2014年11月至2015年10月,采集北京市3个水产品出售市场新鲜扇贝72份。取扇贝消化组织,用聚乙二醇(PEG)8000沉淀法进行病毒富集后提取核酸,用实时荧光逆转录-聚合酶链式反应(RT-PCR)法检测诺如病毒和A组轮状病毒核酸。用半巢式RT-PCR方法扩增诺如病毒GⅠ/GⅡ组衣壳蛋白区基因,对PCR产物进行测序,用BioEdit 7.0.9.0软件进行序列比对,用MEGA 6.06软件构建进化树。结果72份扇贝样品均未检出A组轮状病毒;诺如病毒检出率为27.8%(20/72),其中GⅡ组16份,GⅠ组2份,GⅠ和GⅡ组混合2份。冬季检出率最高,为61.1%(11/18);夏季未检出。8份诺如病毒核酸阳性样品测序结果为GⅡ.17型,属于GⅡ.17型ClusterⅢb分枝。有6株GⅡ.17型毒株与2015年中国人源诺如病毒、2016和2017年日本人源诺如病毒、2017和2018年韩国水中诺如病毒以及2015年日本牡蛎中诺如病毒序列相似性为100.0%。结论北京市市售扇贝中存在诺如病毒污染,食用扇贝有引起病毒性急性胃肠炎的风险。
Objective To monitor the contamination of Norovirus and Rotavirus in scallops sold in Beijing and analyze their genetic characteristics.Methods A total of 72 scallops were collected during November 2014 and October 2015.The digestive tissues of scallops were excised,Norovirus was enriched using the polyethylene glycol(PEG)8000 precipitation method.The virus RNA were extracted and tested for Norovirus and Rotavirus using the real-time reverse transcription-polymerase chain reaction(RT-PCR).The capsid gene of Norovirus was amplified by the semi-nested RT-PCR.PCR products were sequenced directly.Sequence alignment was performed using BioEdit 7.0.9.0 software and the phylogenetic tree was constructed by MEGA 6.06 software.Results Among 72 scallops,Rotavirus was detected negative,and the detection rate of Norovirus was 27.8%(20/72),including 16 strains of genogroup GⅡ,2 strains of genogroup GⅠ,and 2 strains of mixed genogroups GⅠand GⅡ.The highest detection rate of Norovirus(61.1%,11/18)was found in winter,and none was detected in summer.A total of 8 strains were sequenced successfully and identified as GⅡ.17 genotype.These strains all belonged to the ClusterⅢb branch of GⅡ.17 genotype,among which 6 strains shared 100.0%sequence similarity with strains detected in Chinese people in 2015,in Japanese people in 2016 and 2017,in water samples in South Korea in 2017 and 2018,and in oysters in Japan in 2015.Conclusion Norovirus contamination existed in some scallops sold in Beijing,eating uncooked scallops would indicate a risk of viral acute gastroenteritis.
作者
严寒秋
王永全
田祎
靳博
崔海洋
高志勇
王全意
YAN Hanqiu;WANG Yongquan;TIAN Yi;JIN Bo;CUI Haiyang;GAO Zhiyong;WANG Quanyi(Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning,Beijing Center for Disease Prevention and Control,Beijing Research Center for Preventive Medicine,Beijing 100013,China;Xicheng District Center for Disease Prevention and Control,Beijing 100120,China)
出处
《中国食品卫生杂志》
CSCD
北大核心
2020年第4期427-431,共5页
Chinese Journal of Food Hygiene
基金
国家重点研发计划项目(2018YFC1602203-4)。
作者简介
共同第一作者:严寒秋,女,主任技师,研究方向为病原微生物检验,E-mail:13671113791@163.com;共同第一作者:王永全,男,副主任技师,研究方向食品卫生监测,E-mail:wangyq0730@sina.com;通信作者:高志勇,男,研究员,研究方向为传染病监测,E-mail:zhiyonggao1@163.com。
