摘要
目的调查北京市市售结球生菜诺如病毒(norovirus,NoV)和轮状病毒(rotavirus,RV)污染情况。方法2015年11月至2016年10月,在北京市某菜市场摊位采集结球生菜54件。用离心法和直接法,分别对应3种洗脱液洗脱菜叶中病毒并浓缩,用荧光PCR法检测NoV和RV核酸。用半巢式RT-PCR方法扩增NoV阳性样本的衣壳蛋白区基因,PCR产物直接测序,用BioEdit7.0.9.0软件进行序列比对,用MEGA6.06软件构建进化树。结果54件结球生菜,未检出RV。NoV总检出率为11.1%(6/54),其中GI组1件、GⅡ组3件、GI/GⅡ组混合2件。春夏秋冬季检出率依次为8.3%(1/12)、0.0%(0/8)、28.6%(4/14)和5.0%(1/20)。1件GⅡ阳性样本测序成功,为GⅡ.3基因型,该毒株与2014年广东省人源毒株KY348698相似性最高100.0%。结论北京市市售部分结球生菜存在人源NoV污染,生食未洗净结球生菜有引起病毒性急性胃肠炎的风险。
Objective To investigate the contamination of ball lettuce sold by norovirus and rotavirus in Beijing.Methods From November 2015 to October 2016,54 samples were collected at a market stall in Beijing.The potentially existing viruses were eluted and concentrated from the ball lettuce leaves using centrifugation and direct methods corresponding to three kinds of eluants.Norovirus and rotavirus were detected by real time RT-PCR.The partial VP1 gene of norovirus was amplified by semi-nested RT-PCR.The PCR product was sequenced directly,the sequence alignment was performed using the BioEdit 7.0.9.0 software,the phylogenetic tree was constructed with the MEGA 6.06 software.Results Among 54 pieces of ball lettuce,rotavirus was detected negative,and the detection rate of norovirus was 11.11%(6/54).Of these NoV-positive specimens,3(50.0%)were NoV GII,1(16.7%)was NoV GI,and 2(33.33%)were a mix of NoV GI and GII,The detection rates in spring,summer,autumn and winter were 8.3%(1/12),0.0%(0/8),28.6%(4/14)and 5.0%(1/20),respectively.One strain was sequenced successfully and identified as GII.3 genotype.The strain shares 100.0%nucleotide identity with strain KY348698 isolated from human in Guangdong in 2014.Conclusions Human norovirus contamination existed in some ball lettuce sold in Beijing,eating raw unwashed ball lettuce may indicate a risk of viral acute gastroenteritis.
作者
严寒秋
陈艳伟
田祎
李伟红
刘白薇
高志勇
王全意
Yan Hanqiu;Chen Yanwei;Tian Yi;Li Weihong;Liu Baiwei;Gao Zhiyong;Wang Quanyi(Institute for Infectious Disease and Endemic Disease Control,Beijing Center for Disease Control and Prevention,Beijing Research Center for Preventive Medicine,Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning,Beijing 100013,China)
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2020年第2期151-154,共4页
Chinese Journal of Experimental and Clinical Virology
基金
国家重点研发计划(2018YFC1602203-4)。
作者简介
通信作者:高志勇,Email:zhiyonggao1@163.com,电话:010-64407114。
