摘要
为了明确不同连作年限对高粱生长及根际土壤微生物群落代谢结构的影响,采用BIOLOG-ECO微平板方法,研究轮作(CK)、连作5 a(C5)、连作10 a(C10)的高粱生长情况、高粱根际土壤微生物群落代谢结构的变化,以期为高粱连作障碍调控提供理论依据。结果表明,连作显著抑制高粱生长,与CK相比,C5和C10的株高分别显著降低了10.18%、21.28%,且C10对高粱的抑制作用大于C5;C5降低了高粱根际土壤微生物AWCD值,而C10的AWCD值显著增加,在培养96 h时,C10的AWCD值分别是C5和CK的2.05倍和1.47倍;与CK相比,C10高粱根际土壤微生物群落对碳源代谢的多样性及均匀性显著增加,但C5对Shannon多样性指数的影响并不显著;C10和C5均显著增加了高粱根际微生物对羧酸类的代谢活性,分别是CK的2.62倍和2.48倍。因此,高粱不同连作年限根际土壤微生物代谢功能发生变化,随着连作年限的增加高粱根际土壤微生物代谢活性呈现先降低后升高的趋势,这与高粱根系分泌物及根际土壤微生物群落结构的变化有关。
To clarify the effect of different continuous cropping years on the growth of sorghum and the metabolic structure of rhizosphere soil microbial community,the sorghum rhizosphere soil microbial community metabolism structure,considering the regulation of continuous cropping barriers of sorghum as theoretical basis,under the condition of crop rotation(CK),continuous cropping for 5 years(C5)and 10 years(C10)were studied by BIOLOG-ECO microplate method,to provide theoretical basis for continuous cropping obstacle control of sorghum.The results showed that continuous cropping significantly inhibited the growth of sorghum,and compared with CK,the plant height of C5 and C10 significantly reduced by 10.18%and 21.28%,respectively,the inhibitory effect of C10 was more significant than C5.The AWCD value of sorghum rhizosphere soil microbes was reduced under the condition of C5,on the contrary,C10 will significantly increased the AWCD value.The AWCD value of C10 was 2.05 times and 1.47 times of that of C5 and CK with cultivation period of 96 hours,respectively.Compared with CK,the diversity and uniformity of carbon source metabolism in the rhizosphere soil microbial community of C10 sorghum significantly increased,but C5 had no significant effect.Both C10 and C5 significantly increased the utilization capacity of sorghum rhizosphere microorganisms for carboxylic acids than CK by 2.62 times and 2.48 times,respectively.Therefore,the microbial metabolic function of rhizosphere soil will change along with the continuous cropping years,and the microbial metabolic activity of sorghum rhizospheric soil first decreased and then increased with increase of continuous cropping years.In addition,the tendency to go low first and then high is related to the sorghum root exudates and rhizosphere soil microbial community structure changes.
作者
王磊
樊芳芳
白文斌
聂萌恩
江佰阳
王劲松
武爱莲
郭珺
WANG Lei;FAN Fangfang;BAI Wenbin;NIE Meng'en;JIANG Baiyang;WANG Jinsong;WU Ailian;GUO Jun(Institute of Sorghum,Shanxi Agricultural University,Shanxi Key Laboratory of Sorghum Genetic and Germplasm Innovation,Jinzhong 030600,China;College of Resources and Enviroment,Shanxi Agricultural University,Taiyuan 030031,China)
出处
《山西农业科学》
2020年第9期1456-1460,共5页
Journal of Shanxi Agricultural Sciences
基金
“科技助力经济2020”重点专项(SQ2020YFF0419231)
山西省农业科学院重点项目(YGG1646)
高粱遗传与种质创新山西省重点实验室项目(2019Q-2)。
关键词
高粱
连作障碍
不同连作年限
BIOLOG-ECO
根际土壤
sorghum
continuous cropping obstacle
different continuous cropping years
BIOLOG-ECO
rhizosphere soil
作者简介
王磊(1988-),男,山西吕梁人,研究实习员,主要从事高粱栽培技术研究工作;通信作者:白文斌。
