摘要
目的:应用超高效液相色谱-质谱联用分析方法(UPLC-MS),建立葛兰心宁软胶囊中绞股蓝皂苷A的大鼠血浆中的定量分析方法。方法:测定大鼠口服葛兰心宁软胶囊内容物后,血液中绞股蓝皂苷A的血药浓度随时间的动态变化,用药动学统计软件DAS 3.0进行统计,计算药代动力学参数。结果:线性范围为10~500 ng·mL^-1(r=0.9984);高、中、低浓度的日内RSD分别为2.7%、2.6%、4.2%;日间RSD分别为2.4%、2.8%、3.1%;基质效应84.9%~92.1%,RSD<5%;回收率>80.0%。口服葛兰心宁软胶囊,绞股蓝皂苷A在大鼠体内的药代动力学参数t max为0.75 h,t 1/2为(6.247±2.039)h,药时曲线下面积(AUC)为(2562±252)μg·L^-1·h^-1。结论:绞股蓝皂苷A的药动学行为特征为口服快速吸收、达峰时间快、快速消除。
Objective:An in vivo method for the determination of gypenoside A in Glanxinning soft capsules was established by ultra-high performance liquid chromatography-mass spectrometry(UPLC-MS).Methods:The plasma concentration of gypenoside A in rats was determined by UPLC-MS after intragastric administration of Glanxinning soft capsule.The pharmacokinetic parameters of gypenoside A in rats were calculated by DAS 3.0 software.Results:The results showed that the linear range was 10-500 ng·mL^-1(r=0.9984);RSD was 2.7%,2.6%and 4.2%in high,medium and low concentrations;RSD was 2.4%,2.8%and 3.1%in daytime;matrix effect was 84.9%-92.1%,RSD was less than 5%;recovery was more than 80.0%.The oral pharmacokinetic parameters of gypenoside A in rats were T max 0.75 h,t 1/2 of(6.247±2.039)h,and AUC of(2562±252)μg·L^-1·h^-1.Conclusion:The results showed that the pharmacokinetic characteristics of gypenoside A were fast absorption,distribution,peak time and elimination.
作者
胡小虎
孙文军
高昕
王夕静
HU Xiao-hu;SUN Wen-jun;GAO Xin;WANG Xi-jing(Xi′an Chiho Pharmaceutical Co.,Ltd.,Xi′an 710075,China;Xi′an Xintong Pharmaceutical Research Co.,Ltd.,Xi′an 710077,China;School of Pharmacy,Xi′an Jiaotong University,Xi′an 710061,China)
出处
《中国现代中药》
CAS
2020年第7期1066-1071,共6页
Modern Chinese Medicine
作者简介
通信作者:高昕,讲师,研究方向:基于系统生物学的中药化学标识物发现与鉴定,E-mail:gracegao@mail.xjtu.edu.cn。
