摘要
目的探讨葡萄糖转运蛋白3(GLUT3)在子痫前期(PE)胎盘中的表达及意义。方法选取2014年9月至2018年1月海南医学院第二附属医院产科住院治疗的50例PE患者作为PE组,同期选取50例正常妊娠健康孕妇作为对照组进行前瞻性研究。比较两组孕妇的胎盘GLUT3、半胱天冬氨酸蛋白酶(caspase)-3 mRNA、caspase-8 mRNA、caspase-9 mRNA、滋养细胞凋亡指数,并比较轻度和重度PE患者胎盘GLUT3、可溶性血管内皮生长因子受体1(sFlt-1)、血管内皮生长因子(VEGF)表达,采用Pearson相关性分析评价GLUT3与caspase-3、caspase-8、caspase-9、滋养细胞凋亡指数、sFlt-1、VEGF的相关性,以Logistic多元回归方程分析GLUT3、sFlt-1、VEGF与PE病情的相关性。结果PE组孕妇的免疫组化胎盘GLUT3阳性率(90.00%)及GLUT3 mRNA表达(1.16±0.15)均高于对照组(22.00%,0.74±0.10),差异具有统计学意义(P<0.05);PE组孕妇的caspase-3 mRNA(1.56±0.12)、caspase-8 mRNA(1.45±0.10)、caspase-9 mRNA(1.34±0.13)、滋养细胞凋亡指数(0.15±0.03)均高于对照组(0.92±0.06,0.94±0.05,0.91±0.04,0.08±0.02),差异具有统计学意义(P<0.05);GLUT3均与caspase-3(r=0.573)、caspase-8(r=0.529)、caspase-9(r=0.441)、滋养细胞凋亡指数(r=0.607)呈正相关(P<0.05);重度患者胎盘GLUT3 mRNA、sFlt-1 mRNA高于轻度患者,VEGF mRNA低于对照组,差异具有统计学意义(P<0.05);GLUT3与sFlt-1 (r=0.496)呈正相关,与VEGF(r=-0.416)呈负相关(P<0.05)。结论 GLUT3在PE患者胎盘中呈高表达,其水平与滋养细胞凋亡、内皮功能障碍显著相关,并能反映病情的严重程度。
Objective To investigate the expression and significance of glucose transporter 3(GLUT3) in placenta of pre-eclampsia(PE).Methods 50 PE patients who were hospitalized in the obstetrics department of the Second Affiliated Hospital of Hainan Medical College from September 2014 to January 2018 were selected as the PE group,during the same period,50 healthy pregnant women with normal pregnancy were selected as the control group for this prospective study.The placental GLUT3,caspase-3 mRNA,caspase-8 mRNA,caspase-9 mRNA,and trophoblast apoptosis index were compared between the two groups,and the expressions of placenta GLUT3,soluble vascular endothelial growth factor receptor 1(sFlt-1),and vascular endothelial growth factor(VEGF) in patients with mild and severe PE were compared.The correlation between GLUT3 and caspase-3,caspase-8,caspase-9,trophoblast apoptosis index,sFlt-1,and VEGF were analyzed by Pearson correlation analysis,and the correlation between GLUT3,sFlt-1,VEGF and the severity of PE were analyzed by Logistic multiple regression equation.Results The immunohistochemical placenta GLUT3 positive rate(90.00%) and GLUT3 mRNA expression(1.16 ± 0.15) of pregnant women in the PE group were higher than those of the control group(22.00%,0.74 ± 0.10),the difference were statistically significant(P<0.05);caspase-3 mRNA(1.56 ± 0.12),caspase-8 mRNA(1.45 ± 0.10),caspase-9 mRNA(1.34 ± 0.13),trophoblast apoptosis index(0.15 ±0.03) of pregnant women in the PE group were higher than those in the control group(0.92 ±0.06,0.94 ±0.05,0.91 ±0.04,0.08± 0.02),the difference were statistically significant(P< 0.05);GLUT3 was positively correlated with caspase-3(r = 0.573),caspase-8(r =0.529),caspase-9(r =0.441),trophoblast apoptosis index(r =0.607)(P<0.05);the placenta GLUT3 mRNA and sFlt-1 mRNA were higher in severe PE patients than in mild PE patients,and VEGF mRNA was lower than that in control group,the difference were statistically significant(P< 0.05);GLUT3 was positively correlated with sFlt-1(r = 0.496) and negatively correlated with VEGF(r =-0.416)(P<0.05).Conclusion GLUT3 is highly expressed in the placenta of patients with PE,which is significantly associated with trophoblast apoptosis and endothelial dysfunction and can reflect the severity of the disease.
作者
徐秋霞
关红琼
许琳
XU Qiu-xia;GUAN Hong-qiong;XU Lin(Department of Obstetrics,the Second Affiliated Hospital of Hainan Medical College,Haikou Hainan 570311,China)
出处
《临床和实验医学杂志》
2020年第15期1602-1606,共5页
Journal of Clinical and Experimental Medicine
基金
海南省卫生计生行业科研项目(编号:19A200134)。
