摘要
试验旨在探究不同添加量纤维素酶对锦鲤(Cyprinus carpio)抗氧化性能及体色的影响。将360尾初始体质量(53.37±1.44)g的锦鲤随机分为6组,每组3个重复,每重复20尾鱼。其中A组(4%螺旋藻、5%纤维素酶1)、B组(4%螺旋藻、20%纤维素酶1)、C组(4%螺旋藻、5%纤维素酶2)、D组(4%螺旋藻、20%纤维素酶2)、E组(4%螺旋藻)、F组(基础日粮)。结果表明:投喂添加纤维素酶的含螺旋藻饲料明显提升锦鲤红度,显著提升锦鲤皮肤和肌肉类胡萝卜素含量(P<0.05)。纤维素酶处理后,锦鲤肝胰脏、脾脏、血清中超氧化物歧化酶(T-SOD)、过氧化氢酶(CAT)、还原型谷胱甘肽(GSH)、总抗氧化能力(T-AOC)等酶活力明显升高,MDA含量有所降低。纤维素酶可以提高锦鲤对螺旋藻细胞内营养物质的吸收,增强组织抗氧化能力。
The study was to explore the effect of cellulase on antioxidant capacity and body color of Cyprinus carpio.A total of 360 Cyprinus carpio with an initial body mass of(53.37±1.44)g were randomly divided into six groups,including group A(4%Spirulina,5%cellulase 1),group B(4%Spirulina,20%cellulase 1),group C(4%Spirulina,5%cellulose enzyme 2),group D(4%Spirulina,20%cellulose enzyme 2),group E(4%Spirulina)and group F(Basic diet).Each group has three repeats,with 20 fish per repeat.The results showed that:feeding Spirulina containing cellulase significantly increased the redness and carotenoid content of Cyprinus carpio skin and muscle(P<0.05).The antioxidant capacity and body color of Cyprinus carpio were related to the amount of cellulase,but they did not increase with the amount of added.After being treated by cellulase,the enzyme activities of T-SOD,CAT,GSH and T-AOC in liver and pancreas,spleen and serum of Cyprinus carpio were increased,while MDA activity was decreased.It can be seen that cellulase can improve the absorption of nutrients in Spirulina cells of Cyprinus carpio and enhance the antioxidant capacity of tissues.
出处
《饲料研究》
CAS
北大核心
2020年第7期57-61,共5页
Feed Research
基金
天津市水产现代农业产业技术体系创新团队(项目编号:ITTFRS2017014)
天津市科技计划项目(项目编号:19ZXBTSN00140)。
关键词
螺旋藻
锦鲤
纤维素酶
抗氧化
体色
Spirulina
Cyprinus carpio
cellulase
antioxidant
body color
作者简介
侯鹏飞,硕士,研究方向为水产动物增养殖;通信作者:陈成勋,研究员,硕士生导师。
