摘要
目的:探索中药栀子中栀子苷的精制工艺。方法:以栀子苷为指标,在明确跨膜压力差、p H、温度因素影响的基础上,采用Box-Behnken中心组合设计建立数学模型,考察膜截留相对分子量、跨膜压力差、p H,优选栀子提取液的浓缩工艺,并与减压浓缩对比分析,探索纳滤-减压浓缩联用的适用性。结果:优化栀子苷纳滤浓缩工艺为截留相分子量450,跨膜压力差1.20 MPa,p H 7.50,栀子苷截留率为(94.07±1.70)%,与理论截留率92.88%相近,高于减压加热浓缩工艺。纳滤-减压浓缩联用栀子苷转移率可以达到89.27%。结论:选用Box-Behnken响应面法设计优化的栀子苷精制工艺稳定可行,与减压浓缩联用,具有较好的生产适用性。
Objective:To optimization the concentrate process of gardenoside in Zhizi(Gardenia jasminoides).Methods:On the basis of single factor test results,the rejection of geniposide was selected as index,nanofiltration membrane pore size,transmembrane pressure difference and p H were selected as influencing factors during concentrate process by Box-Behnken central composite experiment design.The concentrate efficiency was compared between nanofiltration technique and decompressing concentration.Meanwhile,the applicability of nanofiltration combined with decompressing concentration was also explored.Results:The optimal nanofiltration concentrate conditions were as follows:nanofiltration membrane interception relative molecular weight of 450,transmembrane pressure difference of 1.2 MPa and p H of 7.50,the predicted value of rejection of geniposidic acid was92.88%under the optimum conditions,and the experimental value was(94.07±1.70)%.The separation efficiency of nanofiltration was significantly better than the decompressing concentration.And the transfer rate of gardenoside was 89.27%by the combined technique.Conclusion:The combination of Box-Behnken design and response surface analysis can well optimize the concentrate process of gardenoside by nanofiltration,and the method is stable and feasible.The combined technique of nanofiltration and decompressing concentration has better applicability for heat-sensitive ingredients of traditional Chinese medicine.
作者
宗艳艳
武美蓉
张圆圆
钱雅
魏京璐
ZONG Yan-yan;WU Mei-rong;ZHANG Yuan-yuan;QIAN Ya;WEI Jing-lu(Yangzijiang Pharmaceutical Group Co.,Ltd.,Taizhou Jiangsu 225321,China)
出处
《中医药导报》
2020年第11期54-58,共5页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
关键词
栀子
栀子苷
纳滤
浓缩
响应面分析法
联用技术
Zhizi(Gardenia jasminoides)
geniposide
nanofiltration
concentration
response surface analysis
combined technology
作者简介
通讯作者:宗艳艳,E-mail:tzcxpzq@126.com。
