摘要
目的了解福建省市售预包装食品中致病菌的污染状况。方法根据相关抽检细则收集2017~2019年福建省市售预包装食品8088份食品样品,分析检测金黄色葡萄球菌、单增李斯特氏菌、沙门氏菌、副溶血性弧菌、克罗诺杆菌5种致病菌,并将不合格样品及一部分合格样品进行实时荧光PCR法测试。结果不合格样品共14份,检出率为0.17%(14/8088),其中速冻食品1.80%(9/487);豆制品检出率为0.61%(1/163);肉制品的检出率为0.34%(2/589);婴幼儿配方乳粉第一段0.31%(1/325);调味品检出率0.19%(1/516);实时荧光PCR法检测所有不合格样品均为阳性。结论福建省预包装食品致病菌污染率较低,总体状况良好,速冻食品为主要被污染物,应加强监管;婴幼儿配方乳粉中仍有致病菌检出,仍要严防。由于样品量大,检出率低,建议检验实验室先采用实时荧光PCR法筛选,阳性样品再采用传统微生物法确认,节省人力物力。
Objective To investigate the contamination status of pathogenic bacteria in pre-packaged food on the market in Fujian province.Methods According to the relevant rules of sampling inspection,8088 samples of pre-packaged food sold in Fujian province from 2017 to 2019 were collected.The pre-packaged samples were tested for the contamination with 5 kinds of foodborne pathogens,including Staphylococcus aureus,Listeria monocytogenes,Salmonella,Vibrio parahaemolyticus and Cronobacter spp.,and the unqualified samples and some qualified samples were tested by real-time fluorescent PCR.Results There were 14 unqualified samples with the detection rate of 0.17%(14/8088),including quick-frozen food 1.80%(9/487).The detection rate of soybean products was 0.61%(1/163),the detection rate of meat products was 0.34%(2/589),the first stage of infant formula milk powder was 0.31%(1/325),the detection rate of condiments was 0.19%(1/516).All the unqualified samples tested were positive by real-time PCR.Conclusion The pathogen contamination rate of prepackaged food is low in Fujian,and the overall condition is good.Quick-frozen food is the main be contaminated food and should be strengthened to decrease the occurrence of foodborne infectious isease.The infant formula milk powder still has the pathogenic bacteria detection,should continue to be strengthen the safety supervision.The sample size is large and the detection rate is low.It is suggested that testing laboratory should use the real-time fluorescent PCR method to screen the samples,and then use the traditional microbial method to confirm the positive samples,so as to save manpower and material resources.
作者
林碧莲
柯振华
LIN Bi-Lian;KE Zhen-Hua(Fujian Inspection and Research Institute for Product Quality,National Centre for Quality Supervision and Testing of Processed Food(Fuzhou),Fuzhou 350000,China)
出处
《食品安全质量检测学报》
CAS
2020年第13期4207-4213,共7页
Journal of Food Safety and Quality
基金
福建省科技计划项目(2018Y0019)
福建省产品质量检验研究院科技项目(KY201939A)。
关键词
预包装食品
食源性致病菌
实时荧光PCR
调查分析
prepackaged food
foodborne pathogenic bacteria
real-time PCR
investigation and analysis
作者简介
林碧莲,硕士,工程师,主要研究方向为食品微生物检验检测。E-mail:390005160@qq.com;通讯作者:柯振华,硕士,工程师,主要研究方向为食品微生物检验检测。E-mail:Kzhunter@163.com。
