摘要
壳聚糖为碱性高分子多糖,可广泛应用于各个领域,因此壳聚糖的含量测定至关重要。研究表明,壳聚糖在pH 2.4的Clark-Lubs缓冲溶液中形成质子化的壳聚糖阳离子,再与阴离子染料考马斯亮蓝R250形成离子缔合物,使体系的共振光散射强度显著增强。在325 nm处共振光强度增强值ΔI与壳聚糖浓度在0.1~0.8 mg/L内呈线性关系,检出限(3s/k)为0.012 mg/L。方法用于壳聚糖胶囊的分析,测定值与变色酸2B褪色分光光度法结果一致。
Chitosan is a basic polymer polysaccharide,which can be widely used in various fields,so the determination of chitosan content is very important.The work shows that chitosan forms protonated chitosan cations in Clark-Lubs buffer solution at pH 2.4,which bonded with coomassie brilliant blue R250(anionic dye)could significantly enhance the intensity of resonance light.The resonance light intensity enhancement valueΔI has a linear relationship with the concentration of chitosan within 0.1~0.8 mg/L,and the detection limit(3s/k)was 0.012 mg/L.The method was used for the analysis of chitosan capsules,and the measured values were consistent with the results of discoloring spectrophotometry of chromotrope 2 B.
作者
许钟元
袁莉
李银华
王振
余梦婷
马卫兴
陈丽
XU Zhong-yuan;YUAN Li;LI Yin-hua;WANG Zhen;YU Meng-ting;MA Wei-xing;CHEN Li(School of Marine Life and Fisheries,Jiangsu Ocean University,Lianyungang 222005,China;School of Pharmacey,Jiangsu Ocean University,Lianyungang 222005,China;School of Chemical Engineering,Jiangsu Ocean University,Lianyungang 222005,China;Jiangsu Key Laboratory of Marine Pharmaceutical School Screening,Jiangsu Ocean University,Lianyungang 222005,China;Jiangsu Academy of Marine Resources Development,Jiangsu Ocean University,Lianyungang 222005,China)
出处
《化学试剂》
CAS
北大核心
2020年第7期832-835,共4页
Chemical Reagents
基金
江苏海洋大学一流专业(2019)建设项目(序号12)
淮海工学院研究生创新计划项目(KYCX19-2299)。
作者简介
许钟元(1995-),女,黑龙江齐齐哈尔人,硕士生,主要研究方向为海洋生物大分子;通讯作者:马卫兴,E-mail:3025483196@qq.com。
