摘要
目的:探讨加味四君子汤对Lewis肺癌模型小鼠免疫功能的影响。方法:选取SPF及C57BL/6小鼠60只,10只制备Lewis瘤细胞悬液,40只Lewis肺癌模型小鼠采用随机数字表法分为模型组、环磷酰胺组、加味四君子汤组、联合治疗组,每组各10只,其余10只小鼠为对照组。对比各组之间连续给药14 d后小鼠体质量、肿瘤体积、胸腺质量、胸腺指数、脾细胞内CD4+T百分比、CD8+T百分比、B淋巴细胞百分比。结果:各组小鼠之间肿瘤体积差异有统计学意义(P<0.01);环磷酰胺组、加味四君子汤组、联合治疗组的肿瘤体积均明显小于模型组(P<0.01);联合治疗组的肿瘤体积均明显小于环磷酰胺组、加味四君子汤组(P<0.01);环磷酰胺组与加味四君子汤组肿瘤体积差异无统计学意义(P>0.05)。各组小鼠之间胸腺质量、体质量、胸腺指数差异有统计学意义(P<0.05);对照组小鼠体质量明显高于其它四组(P<0.05);加味四君子汤组的胸腺质量与胸腺指数均明显高于对照组及环磷酰胺组(P<0.05)。各组小鼠之间脾细胞内CD4+T百分比、CD8+T百分比、B淋巴细胞百分比差异均有统计学意义(P<0.01);模型组脾细胞内CD4+T百分比、CD8+T百分比明显低于对照组,B淋巴细胞百分比明显高于对照组(P<0.05);环磷酰胺组、加味四君子汤组脾细胞内CD4+T淋巴细胞、CD8+T淋巴细胞百分比均明显高于对照组,环磷酰胺组、加味四君子汤组脾细胞内B淋巴细胞百分比均明显低于对照组(P<0.05);联合治疗组脾细胞内CD8+T明显高于对照组,B淋巴细胞明显低于对照组(P<0.05);联合治疗组脾细胞内CD4+T百分比、CD8+T百分比明显高于模型组,B淋巴细胞百分比显著低于模型组(P<0.05);联合治疗组脾细胞内CD4+T百分比、CD8+T百分比明显高于环磷酰胺组(P<0.05)。结论:在环磷酰胺治疗基础上加用加味四君子汤可以显著抑制Lewis肺癌肿瘤生长,改善免疫功能。
Objective:To investigate the effect of Jiawei Sijunzi decoction on immune function of Lewis lung cancer model mice.Methods:60 SPF and C57BL/6 mice were selected to prepare Lewis tumor cell suspension.40 Lewis lung cancer model mice were randomly divided into model group,cyclophosphamide group,Jiawei Sijunzi decoction group,combined treatment group with 10 mice in each group,and the other 10 mice as control group.After 14 days of continuous administration,the body mass,tumor volume,thymic mass,thymic index,CD4 T percentage,CD8 T percentage and B lymphocytes percentage in spleen cells were compared.Results:There were significant difference in tumor volume among each group(P<0.01),the tumor volume in cyclophosphamide group,Jiawei Sijunzi decoction group and combined treatment group was significantly smaller than that in model group(P<0.01),and the tumor volume in combined treatment group was significantly smaller than that in cyclophosphamide group and Jiawei Sijunzi decoction group(P<0.01),but there was no significant difference between cyclophosphamide group and Jiawei Sijunzi decoction group(P>0.05).There were significant differences in thymic mass,body mass and thymic index among the mice in each group(P<0.05),the body mass in the control group was significantly higher than that in the other four groups(P<0.05),and the thymic mass and thymic index in the Jiawei Sijunzi decoction group were significantly higher than those in the control group and cyclophosphamide group(P<0.05).There were significant differences in the percentage of CD4+T,CD8+T and the percentage of B lymphocyte in spleen cells among the groups(P<0.01).The percentage of CD4 T,the percentage of CD8 T and the percentage of B lymphocytes in spleen cells in the model group were significantly lower than those in the control group,and the percentage of B lymphocytes in the model group was significantly higher than that in the control group(P<0.05).The percentage of CD4 T and CD8 T in spleen cells of cyclophosphamide group and Jiawei Sijunzi decoction group were significantly higher than those of control group,and the percentage of B lymphocytes was significantly lower than that of control group,and the percentage of CD8 T in spleen cells of combined treatment group was significantly higher than that of control group,and that of B lymphocytes was significantly lower than that of control group.The percentage of CD4 T and CD8 T in spleen cells of the combined treatment group was significantly higher than that of the model group,the percentage of B lymphocytes was significantly lower than that of the model group,and the percentage of CD4 T and CD8 T in the combined treatment group was significantly higher than that of the cyclophosphamide group(P<0.05).Conclusion:On the basis of cyclophosphamide treatment,Jiawei Sijunzi decoction can significantly inhibit the tumor growth of Lewis lung cancer and improve the immune function.
作者
魏丽群
李吉洪
唐凌
WEI Li-qun;LI Ji-hong;TANG Ling(Department of Oncology and Hematology,Leshan Traditional Chinese Medicine Hospital,Leshan 614000,Sichuan,China;Department of Dermatology,Leshan Traditional Chinese Medicine Hospital,Leshan 614000,Sichuan,China)
出处
《川北医学院学报》
CAS
2020年第3期388-391,共4页
Journal of North Sichuan Medical College
基金
四川省卫生厅科研课题(130173)。
关键词
加味四君子汤
肺癌
小鼠
免疫功能
Jiawei Sijunzi decoction
Lung Cancer
Mice
Immune function
作者简介
魏丽群(1983-),女,主治中医师。E-mail:279377729@qq.com。
