摘要
二氢杨梅素和杨梅素是拐枣种子中的重要成分。通过超声辅助法从拐枣种子中提取二氢杨梅素,采用单因素法考察乙醇体积分数、超声辐照功率、提取温度、液料比和超声辐照时间影响参数的基础上,并选用Box-Behnken响应面设计法分析建立了超声辐照功率、超声辐照时间和液料比的二次多项式模型,优化提取工艺。结果得到超声辅助法提取拐枣种子中二氢杨梅素的最佳工艺参数为:乙醇体积分数为60%、超声辐照功率140 W、超声辐照时间30 min、液料比20.5 mL·g-1,提取温度40℃。在此最佳条件下,二氢杨梅素得率为2.14±0.09 mg·g-1。本提取方法简单快速,效率高,有利于拐枣资源的综合加工利用。
Dihydromyricetin is an important component in the seeds of Hovenia dulcis Thunb.The extraction of dihydromyricetin from seeds of H.dulcis by ultrasound-assisted method was carried out on the basis of single factor investigation of ethanol volume fraction,ultrasound irradiation power,extraction temperature,liquid-solid ratio and ultrasound irradiation time.The Box-Behnken response surface design method was used to establish a quadratic polynomial model of ultrasound irradiation power,ultrasound irradiation time and liquid-solid ratio,and optimized extraction process.The optimum conditions for the extraction were as follows:ethanol volume fraction of 60%,ultrasonic irradiation power of 140 W,ultrasonic irradiation time of 30 min,liquid-solid ratio of[JP3]20.5 mL·g-1,and extraction temperature of 40℃.Under these optimal conditions,the yield of[JP]dihydromyricetin was 2.14±0.09 mg·g-1.The extraction method is simple,rapid,and high in efficiency,which is beneficial to the comprehensive processing and utilization of the H.dulcis resources.
作者
张晓南
朱鸿维
赵善舶
白晨
石雅楠
徐浩朋
尹恋
胡淑敏
尹花
ZHANG Xiao-Nan;ZHU Hong-Wei;ZHAO Shan-Bo;BAI Chen;SHI Ya-Nan;XU Hao-Peng;YIN Lian;HU Shu-Min;YIN Hua(State key laboratory of beer biological fermentation engineering,Qingdao beer co.,LTD.,Qingdao 266071;Food Science College,Northeast Agricultural University,Harbin 150036)
出处
《植物研究》
CAS
CSCD
北大核心
2020年第5期775-781,共7页
Bulletin of Botanical Research
基金
黑龙江省自然基金面上项目(C2018025)。
关键词
拐枣
种子
超声辅助提取
二氢杨梅素
响应面
Hovenia dulcis Thunb.
seed
ultrasound-assisted extraction
dihydromyricetin
response surface methodology
作者简介
第一作者:张晓南(1982-),男,副教授,主要从事方向为食品化学方面的研究;通信作者:尹花,E-mail:xiaonanzhang@neau.edu.cn。
