摘要
目的优化翻白草总黄酮制备工艺,并探讨其抗炎活性。方法翻白草经70%乙醇提取、脱醇,上聚酰胺柱进行纯化,以芦丁为对照,分光光度法检测翻白草总黄酮在各工艺单元的分布及纯度;以脂多糖(LPS)诱导单核巨噬细胞RAW264.7,建立炎症筛选模型,用不同浓度(25、50、100μg·mL^-1)的翻白草总黄酮处理后,定量PCR法检测细胞炎症因子一氧化氮合酶(iNOS)、IL^-1β及IL-6的mRNA表达水平。结果聚酰胺层析所得翻白草总黄酮纯度为87.4%,回收率为82.6%;实验剂量(25、50、100μg·mL^-1)下,翻白草总黄酮可显著下调LPS诱导的RAW 264.7细胞炎症因子iNOS、IL^-1β及IL-6的mRNA的表达。结论翻白草总黄酮可用聚酰胺树脂层析制备,其抗炎活性可通过下调iNOS、IL^-1β、IL-6 mRNA的表达来实现。
Objective To study on the preparation process and anti-inflammatory activity of total flavonoids of PotentilladiscolorBge.Methods The plant was extracted with 70%ethanol,and purified with polyamide resin,and rutin was used as a control to investigate the distribution and purity of total flavones.Mononuclear macrophage RAW264.7 was induced with lipopolysaccharide(LPS),and after the treatment with different concentrations(25,50,100μg·mL^-1)of total flavonoids of PotentilladiscolorBge.,quantitative PCR was used to detect the mRNA expression levels of inflammatory factors iNOS,IL^-1β,and IL-6.Results The purity of total flavonoids of PotentilladiscolorBge.obtained by polyamide chromatography was 87.4%,and the yield was 82.6%.The total flavones of PotentilladiscolorBge.could significantly down-regulate the mRNA expression of inflammatory factors iNOS,IL^-1βand IL-6 in RAW 264.7 cells induced by LPS.Conclusion The total flavonoids of PotentilladiscolorBge.can be prepared by polyamide resin chromatography,and its anti-inflammatory activity is related to the down-regulation of mRNA expression of iNOS,IL^-1β,and IL-6.
作者
于泽秋
赵莹
史文杏
曲桂武
YU Zeqiu;ZHAO Ying;SHI Wenxing;QU Guiwu(School of Gerontology,Binzhou Medical University,Yantai 264003,Shandong,P.R.China;Institute of Anti-aging,Binzhou Medical University)
出处
《滨州医学院学报》
2020年第2期132-135,共4页
Journal of Binzhou Medical University
基金
山东省自然科学基金项目(ZR2016HL55)。
关键词
翻白草
总黄酮
制备工艺
抗炎
Potentilladiscolor Bge.
total flavonoid
preparation process
anti-inflammatory
作者简介
通讯作者:曲桂武,E-mail:qu_guiwu@163.com。
