摘要
目的探讨大黄素对人结肠癌CACO-2细胞生长、增殖的影响,并探讨其可能的机制。方法体外培养人结肠癌CACO-2细胞,用不同浓度的大黄素(10,20,40,80μmol·L^-1)处理不同的时间(24,48,72 h)。采用MTT检测结肠癌CACO-2细胞的增殖活力,流式细胞术细胞周期的变化;JC-1检测线粒体膜电位的变化;Western blot检测细胞内ILK(整合素蛋白激酶)、p-PI3K和p-Akt的蛋白水平的表达。结果与空白对照组和DMSO对照组比较,大黄素可显著地抑制CACO-2细胞的增殖,并且存在显著的剂量-时间依赖性(P<0.01);流式细胞术结果表明,大黄素可使细胞的增殖阻滞于G0/G1期;JC-1染色结果显示大黄素可显著降低线粒体膜电位(P<0.01);Western blot结果显示大黄素能够显著降低细胞内ILK、p-PI3K和p-Akt蛋白的表达(P<0.01),且具有浓度-时间依赖性(P<0.01)。结论大黄素对人结肠癌CACO-2细胞表现成明显的抗癌作用,其机制与大黄素抑制ILK/PI3K/Akt信号通路进而抑制癌细胞增殖、诱导细胞周期阻滞于G0/G1期、促进凋亡有关。
OBJECTIVE To observe the effect of emodin on the growth and proliferation of human colon cancer CACO-2 cells and to explore its possible mechanism.METHODS Human colon cancer CACO-2 cells were cultured in vitro and treated with different concentrations of emodin(10,20,40,80μmol·L^-1)for different time(24,48,72 h).MTT was used to detect the proliferative activity of colon cancer CACO-2 cells and the changes of cell cycle were detected by flow cytometry.IC-1 assay was performed for the changes of mitochondrial membrane potentials.Western blot was used to detect the expression of ILK,p-PI3K and p-Akt at protein level.RESULTS Compared with the blank control group and the DMSO control group,emodin treatment significantly inhibited the proliferation of CACO-2 cells in a concentration-time dependent manner(P<0.01).Flow cytometry results showed that emodin blocked the proliferation of CACO-2 cells in G0/G1 phase and JC-1 showed that emodin promoted the decrease of mitochondrial membrane potential(P<0.01).Western blot showed that emodin obviously inhibited the expression of ILK,p-PI3K and p-Akt at protein in the cells(P<0.01).CONCLUSION Emodin obviously exhibited its anti-cancer effect on human colon cancer CACO-2 cells and the potential mechanisms may be that emodin inhibited the proliferation of cancer cells and induced its cell cycle arrest in G0/G1 phase via downregulating ILK/PI3K/Akt signaling pathway.
作者
李中辉
董旺
陈敏
李晓辉
LI Zhonghui;DONG Wang;CHEN Min;LI Xiaohui(Department of Pharmacy,Dazhou Central Hospital,Dazhou,Sichuan 635000,China)
出处
《今日药学》
CAS
2020年第2期116-120,共5页
Pharmacy Today
作者简介
李中辉,药师,研究方向:消化道恶性肿瘤的防治和植物提取物防治肿瘤的机制研究,Tel:13398327653,E-mail:706815742@qq.com。
