摘要
目的分析丹参酮Ⅰ对人胃腺癌细胞SGC-7901真核翻译起始因子2a(EIF2a)、真核翻译起始因子3a(EIF3a)基因表达的调控作用。方法体外培养SGC-7901人胃腺癌细胞株,经丹参酮Ⅰ干预后,采用实时荧光定量聚合酶链反应(RT-PCR)法测定EIF2a和EIF3a mRNA水平;采用四甲基偶氮唑蓝(MTT)比色法测定丹参酮Ⅰ对SGC-7901细胞株增殖的影响,采用流式细胞术检测细胞周期及细胞凋亡率。结果不同质量浓度丹参酮Ⅰ干预后SGC-7901细胞株EIF2a mRNA和EIF3a mRNA降低,与空白对照组比较,差异有统计学意义(P<0.05);EIF2a mRNA与EIF3a mRNA比较,丹参酮Ⅰ1μg/mL>5μg/mL>10μg/mL(P<0.05)。丹参酮Ⅰ1,5,10μg/mL组细胞培养不同时间A值低于空白对照组(P<0.05),抑制率高于空白对照组(P<0.05);不同时间A值比较,丹参酮Ⅰ1μg/mL>5μg/mL>10μg/mL;不同时间抑制率比较,丹参酮Ⅰ1μg/mL<5μg/mL<10μg/mL(P<0.05)。丹参酮Ⅰ不同质量浓度组G1期细胞比例高于空白对照组(P<0.05),S期细胞比例低于空白对照组(P<0.05);G1期细胞比例丹参酮Ⅰ不同质量浓度组对比,丹参酮Ⅰ1μg/mL<5μg/mL<10μg/mL;S期细胞比例丹参酮Ⅰ不同质量浓度组对比,丹参酮Ⅰ1μg/mL>5μg/mL>10μg/mL(P<0.05),丹参酮Ⅰ1,5,10μg/mL组细胞凋亡率均高于空白对照组(P<0.05),不同质量浓度组丹参酮Ⅰ细胞凋亡率比较,丹参酮Ⅰ1μg/mL<5μg/mL<10μg/mL(P<0.05)。结论丹参酮Ⅰ可通过下调人胃腺癌细胞SGC-7901 EIF2a和EIF3a基因表达,抑制癌细胞增殖,促进肿瘤细胞凋亡。
Objective To analyze the regulatory effect of tanshinoneⅠ(TanⅠ)on gene expression of eukaryotic initiation factor 2a(EIF2a)and eukaryotic initiation factor 3a(EIF3a)in human gastric adenocarcinoma cell SGC-7901.Methods The human gastric adenocarcinoma cell lines SGC-7901 were cultured in vitro.After intervention with TanⅠ,the messenger ribonucleic acid(mRNA)levels of EIF2a and EIF3a were determined by quantitative real-time fluorescence polymerase chain reaction(RT-PCR).The effect of TanⅠon the proliferation of SGC-7901 cell lines was determined by methyl thiazolyl tetrazolium(MTT).Cell cycle and apoptosis rate were detected by flow cytometry.Results EIF2a mRNA and EIF3a mRNA were decreased in SGC-7901 cell lines after intervention with different concentrations of TanⅠ;there were statistically significant differences compared with the blank control group(P<0.05).EIF2a mRNA and EIF3a mRNA were the most after intervention with TanⅠof 1μg/mL,followed by 5μg/mL and 10μg/mL(P<0.05).A values of TanⅠ1,5 and 10μg/mL groups after different culture time were lower than those of the blank control group(P<0.05),but the inhibition rates were higher than those of the blank control group(P<0.05).A values at different time were the largestafter intervention with TanⅠof 1μg/mL,follwed by 5μg/mL and 10μg/mL while inhibition rates at different time were the lowest after intervention with TanⅠof 1μg/mL,follwed by 5μg/mL and 10μg/mL(P<0.05).The proportions of cells in G1 phase of different-concentration groups were higher than the blank control group(P<0.05),while the proportions of cells in S phase were lower than the blank control group(P<0.05).The ratio of cells in G1 phase showed TanⅠ1μg/mL<TanⅠ5μg/mL<TanⅠ10μg/mL,and the ratio of cells in S phase showed TanⅠ1μg/mL>TanⅠ5μg/mL>TanⅠ10μg/mL(P<0.05).The apoptosis rates of TanⅠ1μg/mL,5μg/mL and 10μg/mL groups were higher than that of the blank control group(P<0.05).The apoptosis rate under different concentrations of TanⅠshowed TanⅠ1μg/mL<TanⅠ5μg/mL<TanⅠ10μg/mL(P<0.05).Conclusion TanshinoneⅠcan up-regulate the expression of EIF2a gene,inhibit the proliferation of cancer cells and promote the apoptosis of tumor cells through down-regulating the expression of EIF2a and EIF3a in human gastric adenocarcinoma cell lines SGC-7901.
作者
刘新华
刘爱东
LIU Xinhua;LIU Aidong(Qianxi County Hospital of Traditional Chinese Medicine,Tangshan,Heibei,China 064300;Affiliated Hospital of North China University of Technology,Tangshan,Heibei,China 063000)
出处
《中国药业》
CAS
2020年第7期78-82,共5页
China Pharmaceuticals
基金
河北省医学科学研究重点课题[20180793]。
作者简介
第一作者:刘新华,男,大学本科,主治医师,研究方向为胃肠肿瘤学,(电子信箱)buba143454711@163.com。
