摘要
以大麦嫩苗鲜叶为试验材料,对经典蒽酮比色法反应体系中蒽酮及硫酸的用量、反应温度、反应时间、样品提取时间以及叶片取样部位等条件进行优化研究。结果表明,对蒽酮硫酸反应体系进行优化后的条件为:最佳蒽酮用量为100μL、浓硫酸为1 000μL;显色反应温度为100℃,最佳反应时间7 min;样品提取时间以沸水浴10 min为宜;大麦叶片取样部位以倒二叶顶部较为合适。该方法操作简单、快速、实用性强,可为植物样品可溶性糖指标快速筛选提供有效的参考价值。
The content of anthrone and sulfuric acid,reaction temperature,reaction time,sample extraction time and sampling position of leaves in the classical anthrone colorimetric reaction system were optimized by using fresh leaves of young barley seedlings as experimental materials. The results showed that the optimized arthrone and sulphuric acid reaction system consists of 100 μL arthrone and 1 000 μL sulphuric acid,which subjected to color-reaction at 100 ℃ for 7 min. The suitable time for soluble sugars extraction from barley fresh leaf in boiled water was 10 min and the upper section of the second leaf from the top was suggested to be collected as samples. This method is simple,rapid and practical,which provides an effective reference value for the rapid screening of soluble sugar indicators in plant samples.
作者
张述伟
宗营杰
方春燕
黄赛华
李静
许建华
王亦菲
刘成洪
ZHANG Shu-wei;ZONG Ying-jie;FANG Chun-yan;HUANG Sai-hua;LI Jing;XU Jian-hua;WANG Yi-fei;LIU Cheng-hong(Biotechnology Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China;Shanghai Key Laboratory of Agricultural Genetics and Breeding,Shanghai 201106,China;Guangming Seed Industry Company,Shanghai 202171,China)
出处
《食品研究与开发》
CAS
北大核心
2020年第7期196-200,共5页
Food Research and Development
基金
上海市科技兴农重点攻关项目(2017-02-08-00-08-F00074)
国家大麦青稞产业技术体系(CARS-05-01A-02)。
关键词
大麦
可溶性糖
蒽酮法
反应体系
微孔板
barley
soluble sugar
anthrone method
reaction system
microplate
作者简介
张述伟(1994-),男(汉),助理实验师,专科,研究方向:作物种植材料评价选育与指标测定;通信作者:王亦菲(1968-),女(汉),副研究员,本科,研究方向:作物遗传育种;通信作者:刘成洪(1975-),男(汉),研究员,博士,研究方向:作物遗传育种与种质资源评价。
