摘要
采用人工肌肉注射类志贺邻单胞菌(Pseudomonas shigella)后,以针对细菌16S rRNA基因高通量测序技术进行感染鲟鱼肠道菌群组成和多样性分析,结果显示:感染组鲟鱼肠道菌群多样性指数(Chaol、ACE、observed_species、 Shannon和Simpson)均显著低于对照组(P<0.05);感染组和对照组鲟鱼肠道优势菌群均为梭杆菌门(Fusobacteria)、变形菌门(Proteobacteria)、拟杆菌门(Bacteriodia)和厚壁菌门(Firmicute),但感染组鲟鱼肠道拟杆菌门细菌丰度显著小于对照组(P<0.05),而变形菌门(特别是邻单胞菌属)细菌丰度显著大于对照组(P<0.05)。结果表明,类志贺邻单胞菌感染能导致鲟鱼肠道菌群物种多样性和丰度显著变化,为鲟鱼细菌性疾病防控提供了科学依据。
The sturgeons infected with Pseudomonas shigella was used to analyze the intestinal microbiota by high-throughput sequencing.The results that the diversity index(Chaol,ACE,observed_species,Shannon and Simpson) of the intestinal microbiota in the infection group,was significantly lower than that of the control group(P<0.05).The dominant intestinal flora were Fusobacteria,Proteobacteria,Bacteriodia and Firmicute between the infection group and the control group,but the bacterial abundance of Bacteroides in the infected group was significantly lower than that of the control group(P<0.05),while the bacterial abundance of Proteobacteria(especially Proteus) was significantly higher than that of the control group(P<0.05).These results indicated that P.shigelloides infection would cause the significant changes in species diversity and abundance of intestinal microbiota in sturgeons,which provides a scientific basis for the prevention and control of bacterial diseases in sturgeons.
作者
胡安东
张明洋
张飘
杨霞
程振涛
姜海波
文明
HU An-dong;ZHANG Ming-yang;ZHANG Piao;Y ANG Xia;CHENG Zhen-tao;JIANG Hai-bo;WEN Ming(Key Laboratory of Animal Epidemiology and Veterinary Public Health of Guizhou/Research Center of Engineering Technology for Animal Biological Products of Guizhou,College of Animal Science,Guizhou University,Gui yang 550025,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2020年第2期311-317,共7页
Chinese Journal of Veterinary Science
基金
贵州省科技支撑计划资助项目(黔科合支撑[2016]2502号,黔科合支撑[2016]2511号,黔科合支撑[2017]2532号)
贵州省科技平台及人才团队计划资助项目(黔科合平台人才[2018]5253号)
贵州省百层次创新型人才基金资助项目(黔科合人才[2016]4009号)。
关键词
类志贺邻单胞菌
肠道菌群
高通量测序
鲟鱼
Pseudomonas shigella
intestinal microbiota
high-throughput sequencing
sturgeons
作者简介
胡安东(1995-),女,硕士,共同第1作者;张明洋,共同第1作者;通讯作者:姜海波,E-mail:37524501@qq.com;通讯作者:文明,E-mail:as.mwen@gzu.edu.cn。
