摘要
目的研究大豆苷元对人成骨样MG-63细胞分化的影响并对其分子机制进行探讨。方法选取人成骨样MG-63细胞为研究模型,应用不同浓度的大豆苷元对人成骨样MG-63细胞进行处理,培养不同时间对各组细胞分别应用比色法检测碱性磷酸酶(ALP)活性、ELISA法检测Ⅰ型胶原的含量。应用雌激素受体(ER)阻断剂以及小RNA干扰技术,探讨大豆苷元影响人成骨样MG-63细胞分化的分子机制。结果大豆苷元和雌二醇均以时间依赖性的增强细胞中ALP的活性以及Ⅰ型胶原的分泌(P<0.05)。大豆苷元通过增强ALP活性和Ⅰ型胶原分泌促进人成骨样MG-63细胞分化成熟,上述作用可被ER阻断剂ICI 182,780阻断。应用小RNA干扰技术,进一步证实大豆苷元是通过ERα和ERβ共同介导的途径,对MG-63细胞中ALP活性、Ⅰ型胶原的分泌起到调节作用。结论大豆苷元能够促进人成骨样MG-63细胞分化,其作用机制与ER途径有关,由ERα和ERβ共同介导调节。
Objective To investigate the effect of daidzein on differentiation of human osteoblast-like MG-63 cells and its molecular mechanism.Methods Human osteoblast like MG-63 cells were selected as the research model.Different concentrations of daidzein were used to treat human osteoblast like MG-63 cells.Cells of different groups were cultured,and at different time points,colorimetry and ELISA were used to detect the activity of ALP and the content of typeⅠcollagen.Estrogen receptor(ER)blocker and small interference RNA were used to explore the molecular mechanism of daidzein affecting the differentiation of human osteoblast like MG-63 cells,Results Daidzein and estradiol enhanced the activity of ALP and the secretion of typeⅠcollagen in cells in a time-dependent manner(P<0.05).Daidzein could promote the differentiation and maturation of human osteoblast like MG-63 cells by increasing the activity of ALP and typeⅠcollagen,which could be completely blocked by ER blocker ICI 182,780.It was further confirmed that daidzein could regulate ALP activity and typeⅠcollagen secretion in MG-63 cells through ERαand ERβco mediated pathway under using small interfering RNA technology.Conclusion Daidzein can promote the differentiation of human osteoblast-like MG-63 cells.Its mechanism is related to ER pathway,which is mediated by ERαand ERβ.
作者
高丹丹
张玲玲
陈家乐
崔欢欢
温晓昶
王越
金鑫
GAO Dan-dan;ZHANG Ling-ling;CHEN Jia-le;CUI Huan-huan;WEN Xiao-chang;WANG Yue;JIN Xin(College of Pharmaceutical Engineering of Traditional Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Institute of Disaster Medicine,Tianjin University,Tianjin 300071,China;Department of Health Service,Logistics University of Chinese People's Armed Police Forces,Tianjin 300309,China)
出处
《解放军医药杂志》
CAS
2020年第3期10-14,共5页
Medical & Pharmaceutical Journal of Chinese People’s Liberation Army
基金
国家自然科学基金项目(81572852,81273520)
天津市自然科学基金项目(18JCZDJC33200)
武警后勤学院科学技术研究项目(WHJ201712)。
作者简介
通讯作者:金鑫,E-mail:jinxin1227@126.com。
