摘要
目的采用1H核磁共振定量法(quantitative proton nuclear magnetic resonance,qHNMR)测定氢溴酸右美沙芬片中氢溴酸右美沙芬含量。方法 qHNMR以氢溴酸右美沙芬化学位移δ7.05~7.15 ppm处质子峰为定量峰,苯甲酸苄酯δ5.38ppm处为内标峰,在温度为300 K,采样时间为4.01 s,弛豫延迟时间10 s,扫描次数为32次条件下采集混合物qHNMR图谱。比较qHNMR与HPLC的含量测定结果。结果 qHNMR专属性较高,线性范围5.88~23.08mg·mL^-1,回收率为98.4%~99.7%,定量限为0.45 mg·mL^-1,检测限为0.10 mg·mL^-1。qHNMR含量测定结果与HPLC的测定结果基本一致。结论 qHNMR可用于氢溴酸右美沙芬片中氢溴酸右美沙芬的含量测定。
OBJECTIVE To determine the content of dextromethorphan hydrobromide tablets based on quantitative proton nuclear magnetic resonance(qHNMR). METHODS Proton signal peaks at δ 7.05-7.15 ppm of dextromethorphan hydrobromide and δ 5.38 ppm of benzyl benzoate were served as quantitative peaks and internal standard peaks, respectively. The spectra of the mixture were collected at 300 K with 4.01 s acquisition time, 10 s relaxation delay and 32 scanning times. The results of qHNMR and HPLC were also been compared. RESULTS The speciality of qHNMR was very high. There was a linear relationship for dextromethorphan hydrobromide in the range of 5.88-23.08 mg·mL^-1. The recoveries were in the range of 98.4%-99.7%. The limit of quantitation was 0.45 mg·mL^-1 and the limit of detection was 0.10 mg·mL^-1. The content determination results of qHNMR were generally consistent with those of HPLC. CONCLUSION The qHNMR can be used for the quantitative determination of dextromethorphan hydrobromide tablets.
作者
梁丽娜
张晓千
李省
张智强
LIANG Lina;ZHANG Xiaoqian;LI Sheng;ZHANG Zhiqiang(Zhengzhou Shuqing Medical College,Zhengzhou 450064,China;Tianjin Institute of Pharmaceutical Research Pharmaceutical Co.,Ltd.,Tianjin 300301,China)
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2020年第3期304-307,共4页
Chinese Journal of Modern Applied Pharmacy
基金
河南省自然科学基金项目(162300510118)
关键词
氢溴酸右美沙芬片
苯甲酸苄酯
1H核磁共振定量法
dextromethorphan hydrobromide tablets
benzyl benzoate
quantitative proton nuclear magnetic resonance
作者简介
梁丽娜,女,硕士,讲师,Tel:15639718381,E-mail:liangli2019@126.com。
