摘要
目的建立雅连不同炮制品的炮制工艺,测定炮制前后7个生物碱的含量,构建雅连科学的质量控制方法,以提升雅连药材、饮片、精制饮片的质量控制水平。方法通过四因素三水平的正交实验,以生物碱总含量为指标,筛选了酒雅连、姜雅连、萸雅连的最佳炮制工艺;采用高效液相色谱法测定雅连不同炮制品总生物碱以及7个生物碱(小檗碱、黄连碱、巴马汀、药根碱、表小檗碱、非洲防己碱、格陵兰黄连碱)含量。经方法学验证,该方法精密度、重现性、稳定性均符合要求。结果确定了雅连各炮制品的最佳炮制工艺;含量测定结果表明,萸黄连炮制后总生物碱含量最高,姜黄连次之,皆高于生品,7个生物碱含量,因炮制方法不同差别较大。结论雅连各炮制品最佳炮制工艺的确定结合多指标成分定量,可以有效控制雅连药材的质量,为2020年版《中华人民共和国药典》黄连药材质量标准的完善与提高提供参考。
Objective To establish the processing technology of different processed products of Yalian, and to determine the content of 7 alkaloids before and after processing, and construct the scientific quality control method of Yalian to improve the quality control level of Yalian medicine, decoction pieces and refined pieces. Methods Four-factor and three-level orthogonal experiments were carried out to select the best processing technology of Jiu Lian, Jiang Yalian and Yu Yalian based on the total alkaloid content. The high-precision liquid chromatography method was used to determine the total biological products of different products of Yalian and 7 alkaloids(berberine, coptisine, palmatine,jatrorrhizine, epi-berberine, adenine, and greening berberine). The method has proved that the precision, reproducibility and stability of the method meet the requirements. Results The best processing technology of the products of Yalian was confirmed. The content of the samples showed that the total alkaloid content of the scutellaria chinensis was the highest,and the turmeric was the second highest, which was higher than the raw materials and 7 alkaloids. Conclusion The determination of the best processing technology of Yalian’s artillery products combined with multi-index component quantification can effectively control the quality of Yalian medicinal materials, and provide reference for the improvement and improvement of the 2020 edition of the Pharmacopoeia of the People’s Republic of China.
作者
吴晓青
时晓媞
任瑶瑶
蒋合众
魏屹
谭睿
Wu Xiaoqing;Shi Xiaoti;Ren Yaoyao;Jiang Hezhong;Wei Wei;Tan Rui(School of Life Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2019年第8期1649-1655,共7页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家中医药管理局全国第四次中药资源普查（2017PC004）：全国第四次中药资源普查2017年度外业调查研究（沙湾区、犍为县、彭山县），负责人：谭睿
四川省中医药管理局四川中药资源普查成果转化项目（2016年）（2016Z014）：川黄连炮制品的降糖制剂产品开发研制，负责人：谭睿
四川省中医药管理局四川省中医药健康服务项目（2016S20004）：四川省中医药健康服务中医药产业保护规划及宣传，负责人：谭睿
关键词
雅连
炮制品
含量测定
生物碱
Coptidis deltoidea
Processed products
Determination method
Alkaloids
作者简介
通讯作者:谭睿,教授,博士研究生导师,主要研究方向:中药作用机制和质量控制研究。
