摘要
目的探讨丁苯酞对大鼠脑缺血再灌注损伤的保护作用及其相关机制。方法实验分为假手术组、模型组和低、中、高剂量丁苯酞组及全反式维甲酸(ATRA)组。神经功能损伤评分(NDS)评价神经功能,2,3,5-三苯基四唑氯化物(TTC)染色计算缺血脑组织体积,黄嘌呤氧化酶法检测超氧化物歧化酶(SOD),硫代巴比妥酸比色法检测丙二醛(MDA),ELISA检测白介素(IL)-6和肿瘤坏死因子(TNF)-α,Real-time PCR检测血红素加氧酶(HO-1)基因表达,Western blot检测NF-E2相关因子2(Nrf2)和HO-1蛋白表达。结果低、中、高剂量丁苯酞组大鼠NDS评分、脑梗死体积、MDA、IL-6和TNF-α表达水平、HO-1基因2-△△Ct值、Nrf2和HO-1蛋白表达均明显低于模型组(P<0.05)。低、中、高剂量丁苯酞组大鼠SOD明显高于模型组(P<0.05)。低、中、高剂量丁苯酞组大鼠NDS评分、脑梗死体积、MDA、IL-6和TNF-α表达水平、HO-1基因2-△△Ct值、Nrf2和HO-1蛋白表达呈计量依赖性减低,而SOD呈计量依赖性升高(P<0.05)。结论丁苯酞可同过上调Nrf2/HO-1通路发挥抗氧化作用,对脑缺血再灌注大鼠起到神经保护作用。
Objective To observe the effects of butylphthalide on cerebral ischemia-reperfusion injury in rats.Methods We divided 90 SD rats into sham-operation group,model group,low-dose butylphthalide group,medium-dose butylphthalide group,high-dose butylphthalide group,and ATRA group.Neurological impairment score(NDS)was used to evaluate neurological function.TTC staining was used to calculate the volume of ischemic brain tissue.The xanthine oxidase method was used to detect SOD.The thiobarbituric acid colorimetry was used to detect MDA.ELISA was used to detect IL-6 and TNF-αexpressions.The Real-time PCR was used to detect HO-1 gene expression.Western blot was used to detect Nrf2 and HO-1 protein expressions.Results In low-,medium-,and high-butylphthalide groups,the NDS;volume of ischemic brain tissue;expressions of MDA,IL-6 and TNF-α;2-△△Ct value of HO-1;protein expressions of Nrf2 and HO-1 were lower than those in model group(P<0.05),but SOD expression was higher than that in model group(P<0.05).The NDS;volume of ischemic brain tissue;expressions of MDA,IL-6 and TNF-α;2-△△Ct value of HO-1;protein expressions of Nrf2 and HO-1 decreased in a dose-depended manner in low-,medium-,and high-butylphthalide groups,and SOD expression was increased in a dose-depended manner(P<0.05).Conclusion Butylphthalide can play an antioxidant role by up-regulating Nrf2/HO-1 pathway,which benefits neuroprotective function in cerebral ischemia-reperfusion rats.
作者
黄优
陈海云
何超明
庞明武
颜博
王景
李华坚
高唯一
HUANG You;CHEN Haiyun;HE Chaoming;PANG Mingwu;YAN Bo;WANG Jing;LI Huajian;GAO Weiyi(Department of Neurology,Hainan Third People's Hospital,Sanya 572000,China)
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2020年第2期201-205,共5页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
2018年海南省卫生计生行业科研项目(No.18A200030)~~
作者简介
通信作者:陈海云,副主任医师.E-mail:h13518070611@163.com。
