摘要
在关节疾病的发病机制的研究上,目前最常针对的对象还是骨、软骨及滑膜组织,以及从这些组织的整个病理改变的方向去探索可能的发病过程及发病机制,所以存在很多局限性和不准确性,比如忽略了疾病组织内部的单个细胞的异质性在疾病当中所发挥的作用。单细胞测序技术(single-cell sequencing)是在单个细胞水平上对基因组、转录组及表观基因组进行测序的技术,能够从组织样本中获得不同细胞间的异质性信息和对珍贵微量样本进行测序,从而更深层次地反映生命规律与本质,本文就单细胞测序技术的基本方法及在关节疾病方面的应用进行概述。
In the studies of the pathogenesis of joint diseases,the most commonly targeted subjects are bone,cartilage and synovial tissue.At present,it is mainly to explore the possible pathogenesis and pathogenesis from the pathological changes of these tissues.So there are a lot of limitations and inaccuracies,such as ignoring the role of the heterogeneity of individual cells within the disease pathogenesis.Single-cell sequencing is a technique for sequencing genomes,transcriptomes,and epigenomes at a single cell level,enabling to obtain heterogeneous information from different cells and to sequence precious microsamples,even to reflect the laws and essence of life in a deeper level.Here we summary the basic methods of single-cell sequencing technology and its application in joint diseases.
作者
张宇
孙文爽
李寅翠
赵建宁
孟嘉
ZHANG Yu;SUN Wen-shuang;LI Yincui;ZHAO Jian-ning;MENG Jia(Department of Orthopedics,Jinling Hospital,Southern Medical University,Nanjing 210002,China)
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2020年第3期235-238,共4页
Orthopedic Journal of China
基金
江苏省青年医学人才项目(编号：QNRC2016914)
军队医学科技青年培育计划项目(编号：18QNP048)
中国博士后科学基金项目(编号：2017M623359、2018T111167)
关键词
单细胞测序
骨关节疾病
测序技术
single-cell sequencing
bone and joints disease
sequencing technology
作者简介
张宇,硕士研究生,研究方向:关节置换,(电话)13469797041,(电子信箱)441089617@qq.com;通信作者:孟嘉,主治医师,博士研究生,研究方向:运动医学,(电话)13645195753,(电子信箱)michaelmengjia@163.com。
