摘要
目的:研究下肢静脉性溃疡患者血清中基质金属蛋白酶-3(MMP-3)、基质金属蛋白酶抑制剂-1(TIMP-1)及超敏-C反应蛋白(hs-CRP)的表达水平,探讨公英解毒洗剂治疗下肢静脉性溃疡的机制。方法:选取下肢静脉性溃疡患者75例,随机分为对照组25例,复方黄柏液组25例,公英解毒洗剂组25例。于治疗的第1、7、14天抽取患者足背静脉血,检测血清中MMP-3、TIMP-1及hs-CRP表达情况并比较,计算并比较三组的创面愈合率。结果:治疗第7天,创面愈合率和TIMP-1由高到低依次为:公英解毒洗剂组>复方黄柏液组>对照组,差异均有统计学意义;治疗第14天,公英解毒洗剂组和复方黄柏液组的创面愈合率和TIMP-1均高于对照组,差异有统计学意义,但公英解毒洗剂组与复方黄柏液组差异无统计学意义。治疗第7天,血清中hs-CRP和MMP-3水平由高到低依次为:对照组>复方黄柏液组>公英解毒洗剂组,差异均有统计学意义;治疗第14天,复方黄柏液组和公英解毒洗剂组的血清hs-CRP和MMP-3均低于对照组,差异有统计学意义,但公英解毒洗剂组与复方黄柏液组差异无统计学意义。结论:通过对创面的观察及MMP-3、TIMP-1和hs-CRP表达的动态监测,证实公英解毒洗剂有较强的抗菌消炎的功效,值得临床推荐。
Objective Based on the expression of MMP-3 and TIMP-1 in the venous ulcer of the lower extremity,to explore the therapeutic mechanism and target of Gongying Detoxification Lotion(GDL)in the treatment of venous ulcer of lower extremity.Methods In clinical studies,75 patients with venous ulcer of lower limbs were selected.They were randomly divided into 3 groups:control group(n=25),Fufang Huangbai Liquid(FHL)group(n=25)and GDL group(n=25).On the 0,7th,and 14th day,the blood of the dorsal vein of the foot was extracted and the expression of MMP-3 and TIMP-1 was detected by ELISA.The relevant experimental data were measured and the expression of hs-CRP was detected.Results On the 7th day of wound observation in the clinical study group,the detection results of hs-CRP and MMP-3 in serum showed that the expression of hs-CRP and MMP-3 in the three groups at the early stage of inflammatory response increased,among which the expression of hs-CRP and MMP-3 in the control group was significantly higher than that in the GDL group and the FHL group(P<0.01),and the expression of hs-CRP and MMP-3 in the serum of the GDL group and the FHL group was also significantly different(P<0.05).On the 14th day of wound observation in the clinical study group,the expression of hs-CRP and MMP-3 in the serum of GDL group and FHL group was still significantly different from that of the control group(P<0.05).Conclusions The balance regulation mechanism of MMP-3 and TIMP-1 was not only involved in the local inflammatory reaction of ulcer wound but also expressed in the stage of cell proliferation,differentiation and tissue reconstruction during wound healing.
作者
赵波
王雁南
许永楷
梁刚
李涵泊
张玥
陈柏楠
ZHAO Bo;WANG Yan-nan;XU Yong-kai(Department of Peripheral Vascular Disease,the Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250014,China)
出处
《中国中西医结合外科杂志》
CAS
2020年第1期53-57,共5页
Chinese Journal of Surgery of Integrated Traditional and Western Medicine
基金
济南市科技局资助项目(合同号：201705063)
陈柏楠山东省名老中医药专家传承工作室
关键词
下肢静脉性溃疡
公英解毒洗剂
基质金属蛋白酶-3
基质金属蛋白酶抑制剂-1
Venous ulceration of lower extremity
Gongying Detoxification Lotion
matrix metalloproteinase-3
matrix metalloproteinase inhibitor-1
作者简介
通信作者:陈柏楠,E-mail:28198828@qq.com。
