摘要
目的:探讨妊娠期糖尿病肠道变化规律,并分析其临床价值。方法:选取100名妊娠期糖尿病患者、100例正常妊娠人群和100名健康体检女性作为研究对象。收集各组粪便标本,采用粪便细菌基因组DNA提取试剂盒提取粪便肠道菌群DNA,并用16SrDNA测序技术检测肠道菌群;记录妊娠期糖尿病患者血糖相关指标(空腹血糖、空腹胰岛素和胰岛素抵抗指数)和妊娠不良结局。比较各组肠道菌群优势菌群,并分析其与血糖相关指标及妊娠不良结局的关系。结果:共鉴定出9门属肠道内细菌,各组均以厚壁菌门、拟杆菌菌门、变形菌门和放线菌门为主,三组肠道菌群种各优势菌门属比例比较,差异有统计学意义(P<0.05)。经Spearman相关分析,FBG、FINS和IR与厚壁菌门和放线菌门均呈明显正相关(P<0.05),而FBG、FINS和IR与拟杆菌菌门和变形菌门呈明显负相关(P<0.05)。与正常妊娠组比较,糖尿病妊娠组妊娠不良结局总发生率明显升高,差异有统计学意义(P<0.05)。与正常妊娠结局组比较,不良结局发生组肠道菌群中厚壁菌门和放线菌门比例明显升高,而拟杆菌菌门、变形菌门和其它菌门比例则明显下降,差异有统计学意义(P<0.05)。结论:与正常妊娠女性比较,妊娠期糖尿病患者肠道菌群结构及比例改变明显,对其诊断及妊娠不良结局预测有一定价值。
Objective:To investigate the intestinal changes of gestational diabetes mellitus(GDM)and analyze its clinical value.Methods:100 patients with gestational diabetes mellitus,100 normal pregnant women and 100 healthy women were selected as the research objects.Fecal samples were collected,fecal intestinal flora DNA was extracted by fecal bacterial genomic DNA extraction kit,and intestinal flora was detected by 16S rDNA sequencing technology.Blood sugar related indicators(fasting blood sugar,fasting insulin and insulin resistance index)and adverse pregnancy outcomes were recorded in gestational diabetes mellitus patients.The predominant intestinal flora of each group were compared,and the relationship between intestinal flora and blood sugar and adverse pregnancy outcome was analyzed.Results:Nine intestinal bacteria were identified in this study.The dominant bacteria in each group were thick-walled bacteria,bacteroides,Proteus and actinomycetes.The proportion of dominant bacteria in the three groups was significantly different(P<0.05).Spearman correlation analysis showed that FBG,FINS and IR were positively correlated with sclerenchyma and actinomycetes(P<0.05),while FBG,FINS and IR were negatively correlated with Bacteroides and Proteus(P<0.05).Compared with the normal pregnancy group,the total incidence of adverse pregnancy outcomes in the diabetic pregnancy group was significantly higher(P<0.05).Compared with the normal pregnancy outcome group,the proportion of thick-walled and actinomycetes in intestinal flora in the adverse outcome group increased significantly,while the proportion of Bacteroides,Proteus and other flora decreased significantly,the differences were statistically significant(P<0.05).Conclusion:Compared with normal pregnant women,the intestinal flora structure and proportion of gestational diabetes mellitus patients changed significantly,which has certain value in the diagnosis and prediction of adverse pregnancy outcomes.
作者
郑刘芳
刘清秀
李娟
ZHENG Liu-fang;LIU Qing-xiu;LI Juan(Department of Obstetrics,1.Kaizhou District People's Hospital,Chongqing 405425;Chengdu Women's and Children's Central Hospital,Chengdu 610091,Sichuan,China)
出处
《川北医学院学报》
CAS
2019年第6期772-775,共4页
Journal of North Sichuan Medical College
关键词
妊娠期糖尿病
肠道菌群变化
血糖
妊娠不良结局
Gestational diabetes mellitus
Intestinal flora changes
Blood sugar
Adverse pregnancy outcomes
作者简介
郑刘芳(1986-),女,主治医师。E-mail:854851904@qq.com;通讯作者:刘清秀,E-mail:applele007@163.com。
