摘要
以4个绣球品种幼苗为试验材料,采用人工模拟试验,研究了高温干旱胁迫对绣球渗透调节物质含量、抗氧化酶活性、活性氧物质及膜脂过氧化的影响。结果表明:持续高温干旱胁迫条件下,4个绣球品种叶片的可溶性糖含量和MDA含量总体呈升高趋势;POD活性、CAT活性、APX活性、O·2–产生速率和H2O2含量均呈先升高后降低的趋势;可溶性蛋白含量和SOD活性的变化因不同胁迫程度以及不同品种而异;复水后,渗透调节物质含量和MDA含量均有所下降,抗氧化酶活性和活性氧物质均总体升高。通过相关性分析及主成分分析发现:POD、APX、SOD活性以及O2–.产生速率与绣球高温干旱胁迫下的耐受性密切相关。利用隶属函数法进行综合评价,4个绣球品种高温干旱胁迫下耐受性强弱顺序为‘塞布丽娜’>‘无尽夏新娘’>‘粉色梦幻’>‘塔贝’。
The effects of high temperature and drought stress on osmotic adjustment substance content,antioxidant enzyme activities,reactive oxygen species and membrane lipid peroxidation were studied by artificial simulation experiments with four Hydrangea seedlings as experimental materials.The results showed that the soluble sugar content and MDA content of the leaves of four Hydrangea cultivars increased continuously under the condition of high temperature and drought stress.The POD activities,CAT activities,APX activities,O2.–production rate and H2O2 content increased first and then decreased.The changes in soluble protein content and SOD activities varied with different stress levels and different varieties.After rehydration,the content of osmotic adjustment substance and MDA content decreased,and the activities of antioxidant enzymes and active oxygen species increased.Correlation analysis and principal component analysis showed that MDA content,soluble protein content and POD activities were closely related to the tolerance of Hydrangea under high temperature and drought stress.Using the membership function method for comprehensive evaluation,the tolerance of the four Hydrangea cultivars under high temperature and drought stress was in the order of‘Sabrina’>‘Endless Summer The Bride’>‘Pink Fantasy’>‘Taube’.
作者
孙欧文
杨倩倩
章毅
蔡建国
SUN Ou-Wen;YANG Qian-Qian;ZHANG Yi;CAI Jian-Guo(School of Landscape Architecture,Zhejiang Agricultural&Forestry University,Hangzhou 311300,China;Zhejiang A&F University Landscape Architecture Co.,Hangzhou 311300,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2019年第10期1531-1544,共14页
Plant Physiology Journal
基金
国家自然科学基金(31272494)
浙江省花卉团队项目(2011R50034-02)~~
关键词
绣球
干旱胁迫
高温胁迫
生理机制
Hydrangea
drought stress
high temperature stress
physiological mechanism
作者简介
通讯作者:蔡建国,yuanlinzhiwu@163.com。
