摘要
目的:探究白藜芦醇(resveratrol,Res)对高糖诱导成骨细胞增殖、分化的影响及其对沉默信息调节因子(silence information regulator 1,sirt1)/叉头转录因子(forkhead transcription factor 1,FOXO1)通路的影响。方法:分离培养大鼠下颌骨细胞,分为control组、高糖组、高糖+低剂量Res组、高糖+中剂量Res组、高糖+高剂量Res组,细胞计数试剂盒(cell counting kit-8,CCK-8)法检测细胞增殖情况,碱性磷酸酶(alkaline phosphatase,ALP)染色测定ALP活性,Alizarin red S染色检测钙化结节形成,Western blot法检测Ⅰ型胶原(Collagen typeⅠ,CoI)、Runt相关转录因子2(Runt-related transcription factor 2,Runx2)、骨桥蛋白(osteopontin,OPN)、骨钙素(osteocalcin,OCN)蛋白及sirt1/FOXO1通路蛋白表达情况。结果:与control组比较,高糖组成骨细胞增殖抑制率增加,高糖组ALP活性、钙结节沉积、CoI、RUNX2、OPN、OCN蛋白表达水平显著降低(P<0.05);与高糖组比较,白藜芦醇添加组成骨细胞的A值、ALP活性、钙结节沉积、CoI、RUNX2、OPN、OCN蛋白表达水平显著增加(P<0.05),且随着浓度的增加而增加;与control组比较,高糖组成骨细胞sirt1、p27蛋白表达水平显著降低,FOXO1、Bim蛋白表达水平显著升高(P<0.05);与高糖组比较,加入白藜芦醇后sirt1、p27蛋白表达水平明显升高,FOXO1、Bim蛋白表达水平显著降低(P<0.05)。结论:白藜芦醇可能通过调节sirt1/FOXO1通路,改善高糖对成骨细胞增殖、分化的抑制作用。
Objective:To investigate the effects of resveratrol(Res)on the proliferation and differentiation of osteoblasts induced by high glucose and on the silence information regulator(SIRT1)/fork transcription factor(FOXO1)pathway.Methods:Rat mandibular cells were isolated and cultured,and the experiment was divided into control group,high-glucose group,high-glucose+low-dose Res group,high-glucose+medium-dose Res group,and high-glucose+high-dose Res group.CKK-8 assay was used to detect cell proliferation,ALP activity was determined by alkaline phosphatase(ALP)staining,Alizarin red S staining was used to detect the formation of calcified nodules and,Western blotting was used to detect the expressions of type I collagen(COLI)and RUNX2,osteopontin(OPN),osteocalcin(OCN),and SIRT1/FOXO1 pathway protein.Results:Compared with control group,inhibition in high-glucose group increased significantly,ALP activity,calcium nodule deposition,COLI,RUNX2,OPN,and OCN protein levels in high-glucose group decreased significantly(P<0.05).Compared with the high-glucose group,the OD value,ALP activity,calcium nodule deposition,COLI,RUNX2,OPN,and OCN protein levels of osteoblasts in resve-ratrol supplemented groups increased significantly(P<0.05),and increased with the increase of concentration.Compared with control group,the levels of SIRT1 and P27 protein of osteoblasts in high-glucose group decreased significantly,while the levels of FOXO1 and Bim increased significantly(P<0.05).Compared with the high-glucose group,the levels of SIRT1 and P27 protein increased significantly and FOXO1 and Bim protein decreased significantly after adding resveratrol(P<0.05).Conclusion:Resveratrol may improve the inhibitory effect of high glucose on osteoblasts proliferation and differentiation by adjusting SIRT1/FOXO1 pathway.
作者
唐晓悦
朱浩
曹灿
TANG Xiaoyue;ZHU Hao;CAO Can(Department of Stomatology,Peace Branch,General Hospital of the Northern War Zone of the Chinese People's Liberation Army,Shenyang 110000,China;Department of Oral and Maxillofacial Surgery,General Hospital of the Northern War Zone of the Chinese People's Liberation Army,Shenyang 110000,China;Department of Stomatology,General Hospital of the Northern War Zone of the People's Liberation Army,Shenyang 110000,China)
出处
《口腔医学研究》
CAS
北大核心
2020年第1期79-84,共6页
Journal of Oral Science Research
基金
辽宁省科学技术计划项目(编号:201601404)
作者简介
唐晓悦(1988~),女,辽宁沈阳人,硕士,主治医师,研究方向:口腔成骨合和口腔正畸学研究;通信作者:曹灿,E-mail:517592181@qq.com。
