摘要
目的:应用SD大鼠下颌骨来源的原代成骨细胞,观察成骨细胞对甲硝唑和替硝唑的转运,探讨人工种植牙给药的影响及可行性。方法:将原代培养的新生鼠成骨细胞(newbornrat’sosteoblast,N)和成年鼠成骨细胞(adultrat’sosteoblast,A)与溶于PBS的药物共同孵育,分别于1,3,5,10min后,弃去细胞外液,收集各时间点细胞悬液,用高效液相色谱法测定细胞内药物量,用考马斯亮蓝法测定细胞蛋白总量。结果:(1)高效液相色谱法可以准确测定2种药物的量。(2)2种成骨细胞均可将甲硝唑和替硝唑转运至细胞内。结论:成骨细胞具有转运硝基咪唑类药物的能力,证实了人工种植牙给药的可行性。
Objective： To observe the transmembrane transport ofmetronidazole and tinidazole by osteoblast from rat＇ s mandible, and explore the effect and possibility of dental implant administration system. Methods： Co-culture the osteoblasts of newborn（N） or adult（A） rat and agents in PBS at 37℃, remove the extracellular fluid after 1, 3, 5, 10min respectively, collect the suspension, then measure the intra-cellular contents of agents with high performance liquid chromatography（HPLC）, and the cell total protein with Coomassie Brilliant Blue. Results： 1, HPLC is an accurate method for the measurement of the intra-cellular contents of the two agents. 2, Adult and newborn rat＇ s osteoblasts both have the ability of intra-cellular transport of metronidazole and tinidazolet. Conclusions： Osteoblasts from SD rat＇ s mandible can transport metronidazole and tinidazole into cells, which proves the possibility of the dental implant administration system.
出处
《口腔颌面修复学杂志》
2007年第1期59-62,共4页
Chinese Journal of Prosthodontics
关键词
SD大鼠
成骨细胞
甲硝唑
替硝唑
给药途径
种植
人工种植牙
高效液相色谱法
SD rat
osteoblast
metronidazole
tinidazole
route of administration
implant
dental implantation
high performance liquid chromatography
作者简介
通讯作者
