摘要
针对胶质芽孢杆菌SM-01发酵液粘度高,菌液分离困难而导致胞外多糖提取效率低的问题,采用硅藻土吸附-抽滤法,对提取工艺及超滤条件进行了研究,确定了工艺参数。结果表明,发酵液中NaCl添加量为3 g/dL,发酵液稀释倍数为3倍,硅藻土添加量为10 g/L时,在室温下抽滤可除去菌体和蛋白质。滤液在0.1 MPa下经截留相对分子质量为200000的超滤膜超滤纯化,多糖回收率可达73.2%。
Since the broth of Bacillus mucilaginosus SM-01 was of high viscosity and hard to be separated,the diatomite sorption-filtration method was applied to improve the extraction efficiency of exopolysaccharide by optimizing the process and ultrafiltration conditions.The bacteria and protein of the broth can be removed facilely by filtration at room temperature under the optimal conditions included:the addition of NaCl is 3 g/dL,the dilution ratio of the broth is 3 times,and the amount of diatomite is 10 g/L.The filtrate can be purified by ultrafiltration(relative molecular-weight cut-off of 200000)at 0.1 MPa.The recovery of exopolysaccharide was up to 73.2%.
作者
郁莉
付海田
彭梦霞
邓超
陈敬华
YU Li;FU Haitian;PENG Mengxia;DENG Chao;CHEN Jinghua(Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,China;School of Pharmaceutical Science,Jiangnan University,Wuxi 214122,China;Wuxi School of Medicine,Jiangnan University,Wuxi 214122,China)
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2019年第11期78-84,共7页
Journal of Food Science and Biotechnology
基金
国家自然科学基金项目（21574059）
关键词
胶质芽孢杆菌
胞外多糖
硅藻土
提取工艺
超滤
Bacillus mucilaginosus
exopolysaccharide
diatomite
extraction technology
ultrafiltration
作者简介
通信作者:陈敬华(1971-),男,博士,教授,博士研究生导师,主要从事生物制药及活性大分子方面的研究。E-mail:jhchenwhut@126.com。
