摘要
2018年7月,青海省玉树地区一牦牛养殖户2~3月龄的犊牦牛爆发以腹泻为主要症状的疾病。本实验的目的是调查腹泻的病因并了解病原的分子特征。无菌采集9份犊牦牛腹泻粪便样本,采用PCR检测方法对牛冠状病毒、牛轮状病毒、牛病毒性腹泻病毒、沙门氏菌、产肠毒素大肠杆菌、安氏隐孢子虫等6种常见致腹泻病原进行了检测。结果显示,9份样本中有6份为牛冠状病毒阳性,其它5种病原均未检出。对6份阳性样品进行冠状病毒S1亚基基因扩增,序列分析显示,6份样品中冠状病毒S1亚基有7个共同的氨基酸变异,区别于GenBank登录的冠状病毒该段氨基酸序列。系统发育显示6株冠状病毒的S1亚基基因有独特的遗传进化趋势。本实验结果为当地牦牛腹泻的防控提供了参考。
A disease characterized by diarrhea was outbreak in yaks aged 2~3 months old in a farm in Yushu autonomous prefecture of Qinghai Province in July in 2018. The aim of this study was to investigate the cause of diarrhea and to understand the molecular characteristics of the pathogen. Nine yak diarrhea samples were aseptically collected, and 6 common diarrhea pathogens:bovine coronavirus, bovine rotavirus, bovine viral diarrhea virus, enterotoxigenic Escherichia coli, salmonella spp, and Cryptosporidium andersoni were detected by PCR. The results showed that 6 of 9 yak diarrhea samples were positive for bovine coronavirus, and other pathogens were not detected. The S1 subunit gene of 6 bovine coronavirus strains was amplified, and sequence analysis showed that the 6 S1 subunit gene had 7 common amino acid variations compared to all S1 subunits sequences in GenBank database. Phylogenetic analysis showed that the S1 subunit gene of the 6 bovine coronavirus strains showed a unique evolutionary trend. The results provided a reference for the prevention and control of yak diarrhea.
作者
张琳
汤承
ZHANG Lin(Academy of Animal Acience and Veterinary Medicine of Qinghai University,Xining 810016)
出处
《青海畜牧兽医杂志》
2019年第6期6-10,共5页
Chinese Qinghai Journal of Animal and Veterinary Sciences
基金
“十三五”国家重点专项研究计划2016YFD0500907-4
作者简介
通讯作者:汤承(1965-),博士,教授,主要从事青藏高原动物疫病防控研究。E-mail:tangcheng101@163.com。
