摘要
目的研究汉族人群中结直肠癌(colorectal cancer)患者尿苷二磷酸葡醛酸转移酶1A1(UGT1A1)*28基因多态性的分布情况。方法收集2016~2017年合作医疗机构505例汉族结直肠癌患者血液标本,通过焦磷酸测序技术检测UGT1A1*28基因型。根据性别、年龄和地区因素对患者进行分组,统计分析各组间结直肠癌患者UGT1A1*28基因型及等位基因分布情况。结果在505例结直肠癌患者中,UGT1A1*28三种基因型TA6/TA6,TA6/TA7和TA7/TA7频率分别为74.26%,22.97%和2.77%。根据性别分组,患者UGT1A1*28的3种基因型在不同性别结直肠癌患者中的分布差异无统计学意义(χ^2=3.276,P>0.05)。根据年龄分为≤44岁,45~59岁,60~74岁和75~89岁四组,患者UGT1A1*28的3种基因型在不同年龄结直肠癌患者中的分布差异无统计学意义(P>0.05)。根据地区分为山东、辽宁、江苏、上海和甘肃五组,患者UGT1A1*28的3种基因型在不同地区结直肠癌患者中的分布差异无统计学意义(P>0.05)。结论中国汉族结直肠癌患者UGT1A1*28基因多态性主要为TA6/TA6和UGT1A1*28的3种基因型分布与中国汉族结直肠癌患者性别、年龄分层和地区差异无关。
Objective To investigate the distribution of UGT1 A1*28 gene polymorphism in patients with colorectal cancer in Han population. Methods Blood samples from 505 Han patients with colorectal cancer from 2016 to 2017 in cooperative medical institutions were collected, the UGT1 A1*28 genotype was detected by pyrosequencing, and the groups were divided according to gender, age and regional factors. The distribution of UGT1 A1*28 genotypes and alleles in colorectal cancer patients were statistically analyzed. Results In 505 cases of colorectal cancer, the TA6/TA6, TA6/TA7 and TA7/TA7 frequencies of UGT1 A1*28 were 74.26%, 22.97% and 2.77%, respectively. There was no statistically significant in the distribution of UGT1 A1*28 genotypes among patients of different genders with colorectal cancer(χ^2=3.276,P>0.05). According to age grouping ≤ 44 y,45~59 y,60~74 y and 75~89 y, the distribution of UGT1 A1*28 genotypes in patients of different ages with colorectal cancer was no statistically significant(P>0.05). According to the regional grouping of Shandong, Liaoning, Jiangsu, Shanghai and Gansu, the distribution of UGT1 A1*28 genotypes in patients of different regions with colorectal cancer was no statistically significant(P>0.05). Conclusion The UGT1 A1*28 gene polymorphism in Chinese Han colorectal cancer patients was mainly TA6/TA6, and the three genotype distributions of UGT1 A1*28 were not related to gender, age stratification and regional differences in Chinese Han colorectal cancer patients.
作者
滕祥云
王新梦
刘维
樊慧
李琦
TENG Xiang-yun;WANG Xin-meng;LIU Wei;FAN Hui;LI Qi(Changsha Sanji Biotechnology Co.Ltd.,Changsha 410205,China)
出处
《现代检验医学杂志》
CAS
2019年第6期20-23,共4页
Journal of Modern Laboratory Medicine
关键词
结直肠癌
尿苷二磷酸葡醛酸转移酶
基因多态性
colorectal cancer
uridine diphosphate-glucuronosyltransferase 1A1(uGT1A1)
gene polymorphism
作者简介
滕祥云(1980-),男,博士,研发总监,主要从事精准医学基因检测方面的研究,E-mail:1339035044@qq.com。;通信作者:李琦,男,硕士,经理,主要从事医学信息分析工作,E-mail:liqi@3gbio.com.cn。
