摘要
目的探讨性别对新生小鼠缺氧缺血性脑损伤后学习记忆能力的影响。方法选取50只日龄为10 d的新生C57BL/6小鼠,根据随机数字表法分为缺氧缺血组以及假手术组,缺氧缺血组共28只小鼠,假手术组共22只小鼠,每组均雌雄各半。缺氧缺血组进行左侧颈总动脉结扎后置于含有8%氧气和92%氮气混合气体的34℃缺氧箱中缺氧45 min。假手术组仅切开左侧颈部皮肤并缝合。2个月后,分别用Y迷宫实验和Morris水迷宫实验评估小鼠的学习和记忆能力。结果缺氧缺血组建模成功率为71.4%(20/28),假手术组建模成功率为100.0%(22/22),共42只小鼠进入实验。在Y迷宫实验中,缺氧缺血组的新异臂进入次数和新异臂总路程与假手术组比较,差异均有统计学意义(F=16.068,P<0.001;F=8.532,P=0.007)。同性别中组间比较,缺氧缺血组的新异臂进入次数(雄性:P=0.001;雌性:P=0.012)和新异臂总路程(雄性:P=0.010;雌性:P=0.046)明显低于假手术组。同一组别雌雄间比较,缺氧缺血组雌性的新异臂进入次数(P=0.039)和新异臂总路程(P=0.043)均高于雄性。在Morris水迷宫实验中,缺氧缺血组在定位航行实验中的逃避潜伏期高于假手术组,且雄性表现更明显(P<0.001);在空间探索实验中,缺氧缺血组和假手术组目标区域停留时间(F=8.297,P<0.001)和目标区域进入次数(F=4.042,P=0.014)比较,差异均有统计学意义。同性别中组间比较,缺氧缺血组目标区域停留时间(雄性:P=0.003;雌性:P=0.038)与目标区域进入次数(雄性:P=0.006;雌性:P=0.041)低于假手术组。同一组别雌雄间比较,缺氧缺血组雄性的目标区域停留时间(P=0.018)和目标区域进入次数(P=0.032)低于雌性。结论缺氧缺血性脑损伤对新生小鼠学习记忆能力可能产生轻度损害,其对不同性别小鼠学习记忆能力的影响有明显差别,对雄性的影响高于雌性。
Objective To investigate the effect of sex on learning and memory ability of newborn mice with hypoxic-ischemic brain injury. Methods Fifty C57 BL/6 mice aged 10 days were divided into hypoxia-ischemia group and sham group according to the random number table method, and there were 28 in the hypoxic-ischemic group and 22 in the sham group with half female and half male respectively. In the ischemia-hypoxia group, the left common carotid artery was ligated and then the mice were placed in 34℃ hypoxia chambers with 8% oxygen and 92% nitrogen mixture for45 minutes. In the sham group, only the skin of the left neck was cut and sutured. After 2 months, Y maze test and Morris water maze test were used to evaluate the learning and memory ability of mice. Results The success rate of the hypoxicischemic group was 71.4%(20/28), and that of the sham group was 100.0%(22/22), a total of 42 mice were enrolled in the experiment. In Y maze test, there were differences in entries and total distance of new arms between the two groups(entries: F=16.068, P<0.001;total distance: F=8.532, P=0.007);compared between different groups in the same gender, the entries and total distance of new arms in the hypoxic-ischemic group were lower than those in the sham group with statistically significant differences(entries in males: P=0.001, entries in females: P=0.012;total distance in males: P=0.010,total distance in females: P=0.046). Compared between males and females in the same group, the entries and total distance of new arms of females were higher than those of males in the hypoxic-ischemic group, with statistically significant differences(P=0.039, 0.043). In Morris water maze test, the escape latency of positioning navigation in the hypoxicischemic group was higher than that in the sham group, and males showed more obviously poor performance(P<0.001);in the experiment of space exploration, differences were found in the duration of stay and the target quadrant entries between the two groups(duration of stay: F=8.297, P<0.001;entries: F=4.042, P=0.014), and there were statistically significant differences in the same gender males and females in the hypoxic-ischemic group and the sham group(duration of stay in males: P=0.003, duration of stay in females: P=0.038;entries in males: P=0.006, entries in females: P=0.041).Compared between males and females in the same group, the duration of stay and the target quadrant entries of females were higher than those of males in the hypoxic-ischemic group, with statistically significant differences(duration of stay:P=0.018;entries: P=0.032). Conclusions The learning and memory ability of newborn mice may be slightly impaired after hypoxic ischemic brain injury. There is significant difference in the effect on learning and memory ability between different genders, and the effect on males is higher than that on females.
作者
林爱金
王洁琼
易沙沙
敖丽娟
陈茉弦
LIN Aijin;WANG Jieqiong;Yl Shasha;AO Lijuan;CHEN Moxian(College of Rehabilitation,Kunming Medical University,Kunming,Yunnan 650500,P.R.China)
出处
《华西医学》
CAS
2019年第12期1412-1416,共5页
West China Medical Journal
基金
云南省教育厅科学研究基金(2018Y044,2019Y038)
作者简介
通信作者:敖丽娟,Email:13508710081@qq.com;通信作者:陈茉弦,Email:37774290@qq.com。
