摘要
目的观察跑轮训练对大脑中动脉闭塞(MCAO)脑缺血再灌注损伤(CIRI)模型大鼠神经功能及肢体运动协调性的影响。方法SPF级雄性SD大鼠30只采用随机数字表法均分为假手术组、MCAO组、跑轮组,每组各10只。造模以后,假手术组存活10只,MACO组存活7只,跑轮组存活8只。跑轮组进行跑轮训练;治疗于术后第2天进行,治疗共4周,5次/周。假手术组和MCAO组不予治疗。采用经皮层电刺激记录运动诱发电位(MEP)并结合Zea Longa神经学评分评价神经功能;采用强迫游泳实验(FST)评估大鼠的绝望状态;采用悬吊和腓肠肌张力实验评估肢体肌力;采用斜板实验、足误实验和Grip test评分评估神经功能与躯体平衡及运动功能;采用伊文思蓝渗透法观察血脑屏障通透性。结果与MCAO组比较,跑轮组Zea Longa评分、不动时间、MEP各时相点潜伏期及伊文思蓝含量降低,而足误实验评分、Grip test评分、挣扎时间、悬吊实验评分、最大倾斜角度、等长收缩肌力及MEP各时相点波幅峰值等提高,差异有统计学意义(均P<0.05)。结论跑轮训练可促进MCAO模型大鼠神经及运动功能恢复,其机制可能与跑轮训练改变血脑屏障通透性、增加骨骼肌肌力并提高肢体运动协调性有关。
Objective To observe the effect of running wheel training on the body motor coordination and nerve function in cerebral ischemiac reperfusion injury(CIRI)model rats with middle cerebral artery occlusion(MCAO).Methods 30 SPF male SD rats were divided into sham operation group,MCAO group and running wheel group by random number table method,with 10 rats in each group.After modeling,10 animals survived in sham operation group,7 animals survived in MACO group,and 8 animals survived in running wheel group.The running wheel group performed running wheel training,the treatment was carried out on the second day after the operation,treatment lasted for 4 weeks,5 times/week.The sham operation group and MCAO group were not treated.Transcortical electrical stimulation was used to record motor evoked potential(MEP)and combined with Zea Longa neurological score to evaluate nerve function,and forced swimming test(FST)was used to evaluate the desperate state of rats.Suspension and gastrocnemius muscle tension test were used to evaluate the muscle strength of the limbs.The incline plane test,foot error experiment and Grip test score were used to evaluate the nerve function,body balance and motor function.Evans blue penetration method was used to observe the blood-brain barrier permeability.Results Compared with the MCAO group,the Zea Longa score,immobility time,MEP latency at each time point and Evans blue content decreased,foot error experiment score,Grip test score,struggling time,suspension experiment score,maximum inclination angle,isometric contraction muscle strength and peak amplitude of MEP at each time point all increased,and the differences were statistically significant(all P<0.05).Conclusion Running wheel training can promote the recovery of nerve and motor function in MCAO model rats,and its mechanism may be related to the change of blood-brain barrier permeability and improvement of skeletal muscle strength and limb motor coordination.
作者
范荣国
廖恒
穆敬平
李又春
Fan Rongguo;Liao Heng;Mu Jingping;Li Youchun(Health Management Center,Danjiangkou First Hospital,Shiyan 442700,China;Sleep Psychosomatic Medical Center,Taihe Hospital(Affiliated Hospital of Hubei University of Medicine),Shiyan 442000,China)
出处
《心脑血管病防治》
2023年第2期12-16,共5页
CARDIO-CEREBROVASCULAR DISEASE PREVENTION AND TREATMENT
基金
湖北省卫生健康委科研项目(ZY2021M006)
关键词
脑缺血再灌注损伤
跑轮训练
血脑屏障通透性
骨骼肌
神经及运动功能
协调性
Cerebral ischemia-reperfusion injury
Running wheel training
Blood brain barrier permeability
Skeletal muscle
Nerve and motor function
Coordination
作者简介
通信作者:李又春,Email:ZouWeilab@163.com
